Cloned (Comment) | Organism |
---|---|
- |
Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0426 | - |
5'-hydroxyaverantin | pH 7.5, 37°C | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25873 | - |
x * 25873, calculated from sequence, TOF MS analysis | Levilactobacillus brevis |
33000 | - |
x * 33000, SDS-PAGE | Levilactobacillus brevis |
100000 | - |
gel filtration | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis | the enzyme catalyzes a reaction in aflatoxin biosynthesis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
5'-hydroxyaverantin + NADP+ | Levilactobacillus brevis IFO 12005 | the enzyme catalyzes a reaction in aflatoxin biosynthesis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
- |
- |
Levilactobacillus brevis IFO 12005 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Levilactobacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.552 | - |
pH 7.5, 37°C | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-hydroxyaverantin + NADP+ | the enzyme catalyzes a reaction in aflatoxin biosynthesis | Levilactobacillus brevis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
5'-hydroxyaverantin + NADP+ | a mixture of two diastereomers, (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin | Levilactobacillus brevis | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
5'-hydroxyaverantin + NADP+ | the enzyme catalyzes a reaction in aflatoxin biosynthesis | Levilactobacillus brevis IFO 12005 | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
5'-hydroxyaverantin + NADP+ | a mixture of two diastereomers, (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin | Levilactobacillus brevis IFO 12005 | (1'S)-5'-oxoaverantin + NADPH + H+ | - |
? | |
additional information | no activity with nidurufin, averantin, and 6-phenylhexal-1-ol | Levilactobacillus brevis | ? | - |
? | |
additional information | no activity with nidurufin, averantin, and 6-phenylhexal-1-ol | Levilactobacillus brevis IFO 12005 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Levilactobacillus brevis |
? | x * 25873, calculated from sequence, TOF MS analysis | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
Lac-HAVN dehydrogenase | - |
Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Levilactobacillus brevis |
40.55 | - |
- |
Levilactobacillus brevis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | 35°C: about 65% of maximal activity, 60°C: about 30% of maximal activity | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Levilactobacillus brevis |
7.5 | - |
assay at | Levilactobacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 75% of maximal activity, pH 8.5: about 30% of maximal activity | Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | no activity with NAD+ | Levilactobacillus brevis |