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Literature summary for 1.2.1.95 extracted from
- Site-directed mutational analysis of the novel catalytic domains of alpha-aminoadipate reductase (Lys2p) from Candida albicans (2003), Mol. Genet. Genomics, 269, 271-279.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant enzymes expressed in Escherichia coli BL21 | Candida albicans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A459G | 106% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| A475G | 109% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| A979G | 94% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| D461E | 4% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| D461N | no alpha-aminoadipate reductase activity | Candida albicans |
| D466E | 15% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| E420T | 101% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| F415Y | 71% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| F452L | 6% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| F452V | 45% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| F468L | 96% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| F982L | 4% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| F982V | no alpha-aminoadipate reductase activity | Candida albicans |
| G418A | 31% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| G418V | no alpha-aminoadipate reductase activity | Candida albicans |
| G421A | 75% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| G425A | 99% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| G474A | 41% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| G978A | 2% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| G978S | no alpha-aminoadipate reductase activity | Candida albicans |
| G981A | no alpha-aminoadipate reductase activity | Candida albicans |
| G981V | no alpha-aminoadipate reductase activity | Candida albicans |
| G984A | 3% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| G984S | no alpha-aminoadipate reductase activity | Candida albicans |
| H460R | no alpha-aminoadipate reductase activity | Candida albicans |
| I422V | 92% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| I463L | no alpha-aminoadipate reductase activity | Candida albicans |
| I987V | 92% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| K424R | 26% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| K424T | 3% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| K451Q | 58% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| K451R | no alpha-aminoadipate reductase activity | Candida albicans |
| K535Q | 94% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| L446I | 71% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| L471V | 89% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| L983V | 31% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| M440L | 99% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| P423V | 51% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| P462A | 2% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| P462S | no alpha-aminoadipate reductase activity | Candida albicans |
| Q464E | 17% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| Q511E | 31% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| Q511L | 129% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| R465K | 2% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| R465S | no alpha-aminoadipate reductase activity | Candida albicans |
| S417A | 10% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| S417T | 82% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| S419A | 16% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| S419T | no alpha-aminoadipate reductase activity | Candida albicans |
| S456T | 59% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| S985A | 85% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| T416A | no alpha-aminoadipate reductase activity | Candida albicans |
| T416S | 35% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| T469S | 80% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| T506A | 49% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| T506S | no alpha-aminoadipate reductase activity | Candida albicans |
| T534A | 103% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| T977S | 70% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| T980S | 28% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| V426L | 64% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
| V529I | 117% of the alpha-aminoadipate reductase activity of wild-type enzyme | Candida albicans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| MgCl2 | required | Candida albicans |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | Q12572 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate | presence of ATP and Mg2+ required | Candida albicans | L-2-aminoadipate + NADPH + H+ + ATP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-aminoadipate reductase | - |
Candida albicans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Candida albicans | |
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