Literature summary for 1.2.1.95 extracted from

Ehmann, D.E.; Gehring, A.M.; Walsh, C.T.
Lysine biosynthesis in Saccharomyces cerevisiae: mechanism of alpha-aminoadipate reductase (Lys2) involves posttranslational phosphopantetheinylation by Lys5 (1999), Biochemistry, 38, 6171-6177.

Search for more literature for 1.2.1.95

Cloned(Commentary)

Cloned (Comment)	Organism
expression in ERscherichia coli	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.43	-	S-carboxymethyl-L-cysteine	pH 8.8, 37°C	Saccharomyces cerevisiae
0.47	-	L-2-aminoadipate	pH 8.8, 37°C	Saccharomyces cerevisiae
0.62	-	NADPH	pH 8.8, 37°C	Saccharomyces cerevisiae
2.7	-	D-2-aminoadipate	pH 8.8, 37°C	Saccharomyces cerevisiae
10	-	adipate	pH 8.8, 37°C	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
155000	-	x * 155000, calculated	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P07702	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
side-chain modification	residue Ser880 is modified by phosphopantetheinylate, leading to activiation of the enzyme. Protein Lys5 catalyzes the modification using coenzyme A	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adipate + NADPH + H+ + ATP	-	Saccharomyces cerevisiae	adipate semialdehyde + NADP+ + AMP + diphosphate	-	?
D-2-aminoadipate + NADPH + H+ + ATP	-	Saccharomyces cerevisiae	(R)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate	-	?
L-2-aminoadipate + NADPH + H+ + ATP	-	Saccharomyces cerevisiae	(S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate	-	?
S-carboxymethyl-L-cysteine + NADPH + H+ + ATP	-	Saccharomyces cerevisiae	S-formylmethyl-L-cysteine + NADP+ + AMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 155000, calculated	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
LYS2	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0045	-	adipate	pH 8.8, 37°C	Saccharomyces cerevisiae
0.63	-	D-2-aminoadipate	pH 8.8, 37°C	Saccharomyces cerevisiae
6	-	S-carboxymethyl-L-cysteine	pH 8.8, 37°C	Saccharomyces cerevisiae
41.7	-	L-2-aminoadipate	pH 8.8, 37°C	Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.0005	-	adipate	pH 8.8, 37°C	Saccharomyces cerevisiae
0.23	-	D-2-aminoadipate	pH 8.8, 37°C	Saccharomyces cerevisiae
14	-	S-carboxymethyl-L-cysteine	pH 8.8, 37°C	Saccharomyces cerevisiae
88.3	-	L-2-aminoadipate	pH 8.8, 37°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)