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Literature summary for 1.2.1.9 extracted from
- Purification and kinetic and structural properties of spinach leaf NADP-dependent nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase (1988), Arch. Biochem. Biophys., 260, 830-840.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-3-phosphoglycerate | competitive | Spinacia oleracea |  |
| Diamide | - |
Spinacia oleracea | |
| iodoacetamide | - |
Spinacia oleracea | |
| L-Glyceraldehyde 3-phosphate | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
NADP+ | - |
Spinacia oleracea | |
| 0.031 | - |
D-glyceraldehyde 3-phosphate | - |
Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 190000 | 200000 | gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | Spinacia oleracea | may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm | D-3-phosphoglycerate + NADPH | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + H+ | proposed mechanism | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15 | - |
- |
Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 50 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 10 mM mercaptoethanol, at least 6 months, or precipitated in 60% ammonium sulfate | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glyceraldehyde-3-phosphate + NADP+ | absolutely specific for D-isomer | Spinacia oleracea | D-3-phosphoglycerate + NADPH | - |
ir | |
| D-glyceraldehyde-3-phosphate + NADP+ | may be part of a system that is responsible for the export of photosynthetically generated NADPH from chloroplast to cytoplasm | Spinacia oleracea | D-3-phosphoglycerate + NADPH | - |
ir | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | absolutely specific for | Spinacia oleracea | |
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