Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermus thermophilus |
expression in Escherichia coli | Deinococcus radiodurans |
expression in Escherichia coli | Halalkalibacterium halodurans |
expression in Escherichia coli | Bacillus licheniformis |
Crystallization (Comment) | Organism |
---|---|
- |
Bacillus licheniformis |
mutants R100A crystallizes in the same lattice as wild-type, mutant R100A/K104A/R111A crystaLLIZES IN space group P1 | Thermus thermophilus |
space group P21 with eight molecules in the asymmetric unit | Halalkalibacterium halodurans |
Protein Variants | Comment | Organism |
---|---|---|
K104A | mutation in alpha3 helix which participates in the dimer-dimer interaces. Catalytic efficiency similar to wild-type, mutant forms a hexamer | Thermus thermophilus |
R100A | mutation in alpha3 helix which participates in the dimer-dimer interaces. Catalytic efficiency similar to wild-type, contrary to wild-type, the mutant forms a dimer | Thermus thermophilus |
R100A/K104A/R111A | mutation in alpha3 helix which participates in the dimer-dimer interaces. Catalytic efficiency similar to wild-type, contrary to wild-type, the mutant forms a dimer | Thermus thermophilus |
R102A | mutation in alpha3 helix which participates in the dimer-dimer interaces. Catalytic efficiency similar to wild-type, contrary to wild-type, the mutant forms a dimer | Deinococcus radiodurans |
R111A | mutation in alpha3 helix which participates in the dimer-dimer interaces. Catalytic efficiency similar to wild-type, mutant forms a hexamer | Thermus thermophilus |
R153A | mutation in alpha3 helix which participates in the dimer-dimer interaces. Catalytic efficiency similar to wild-type, mutant forms a hexamer | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
119000 | - |
dynamic light scattering | Halalkalibacterium halodurans |
119000 | - |
dynamic light scattering | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q65NN2 | - |
- |
Bacillus licheniformis DSM 13 | Q65NN2 | - |
- |
Deinococcus radiodurans | Q9RW56 | - |
- |
Deinococcus radiodurans DSM 20539 | Q9RW56 | - |
- |
Halalkalibacterium halodurans | Q9K9B2 | - |
- |
Halalkalibacterium halodurans DSM 18197 | Q9K9B2 | - |
- |
Thermus thermophilus | Q72IB9 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72IB9 | - |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallization data | Bacillus licheniformis |
dimer | dynamic light scattering and crystallization data | Halalkalibacterium halodurans |
hexamer | protein exists primarily as a trimer-of-dimers hexamer in solution, small-angle X-ray scattering and crystallization data | Thermus thermophilus |
hexamer | protein exists primarily as a trimer-of-dimers hexamer in solution, small-angle X-ray scattering and crystallization data | Deinococcus radiodurans |
Synonyms | Comment | Organism |
---|---|---|
DR_0813 | - |
Deinococcus radiodurans |