Literature summary for 1.2.1.88 extracted from

Pemberton, T.A.; Srivastava, D.; Sanyal, N.; Henzl, M.T.; Becker, D.F.; Tanner, J.J.
Structural studies of yeast delta(1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1): active site flexibility and oligomeric state (2014), Biochemistry, 53, 1350-1359.

Search for more literature for 1.2.1.88

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens
expression in Escherichia coli	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme and in complex with NAD+, to 1.95 and 2.17 A resolution, respectively	Saccharomyces cerevisiae
the substrate-free form displays multiple active site conformations. Protein forms a trimer-of-dimers hexamer in solution	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
W193A	mutation disrupts hexamer formation, mutant forms a dimer	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.104	-	1-pyrroline-5-carboxylate	pH 7.5, 20°C	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P30038	-	-
Saccharomyces cerevisiae	P07275	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-pyrroline-5-carboxylate + NADH + H+	-	Saccharomyces cerevisiae	L-proline + NAD+	-	?

Subunits

Subunits	Comment	Organism
hexamer	crystallization data	Saccharomyces cerevisiae

Synonyms

Synonyms	Comment	Organism
Aldh4a1	-	Homo sapiens
Aldh4a1	-	Saccharomyces cerevisiae
Put2	-	Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.5	-	1-pyrroline-5-carboxylate	pH 7.5, 20°C	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
14	-	1-pyrroline-5-carboxylate	pH 7.5, 20°C	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)