Crystallization (Comment) | Organism |
---|---|
unligandend form and in complex with NAD+, NADPH, and with product glutamate | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
1-pyrroline-5-carboxylate | pH 7.4, 50°C | Thermus thermophilus | |
0.071 | - |
NAD+ | pH 7.4, 50°C | Thermus thermophilus | |
0.12 | - |
NADP+ | pH 7.4, 50°C | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Thermus thermophilus | L-glutamate + NADH + H+ | - |
ir | |
1-pyrroline-5-carboxylate + NADP+ + H2O | NAD+ is preferred over NADP+ | Thermus thermophilus | L-glutamate + NADPH + H+ | - |
ir | |
additional information | no reverse reaction using L-glutamate as substrate within pH 5.5 to pH 10.0 | Thermus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | consisting of three dimers, crystallization data | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
NADP+ | pH 7.4, 50°C | Thermus thermophilus | |
1.6 | - |
NAD+ | pH 7.4, 50°C | Thermus thermophilus | |
1.6 | - |
1-pyrroline-5-carboxylate | pH 7.4, 50°C | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | broad | Thermus thermophilus |