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Literature summary for 1.2.1.88 extracted from

  • Inagaki, E.; Ohshima, N.; Takahashi, H.; Kuroishi, C.; Yokoyama, S.; Tahirov, T.H.
    Crystal structure of Thermus thermophilus DELTA1-pyrroline-5-carboxylate dehydrogenase (2006), J. Mol. Biol., 362, 490-501.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
unligandend form and in complex with NAD+, NADPH, and with product glutamate Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
1-pyrroline-5-carboxylate pH 7.4, 50°C Thermus thermophilus
0.071
-
NAD+ pH 7.4, 50°C Thermus thermophilus
0.12
-
NADP+ pH 7.4, 50°C Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD+ + H2O
-
Thermus thermophilus L-glutamate + NADH + H+
-
ir
1-pyrroline-5-carboxylate + NADP+ + H2O NAD+ is preferred over NADP+ Thermus thermophilus L-glutamate + NADPH + H+
-
ir
additional information no reverse reaction using L-glutamate as substrate within pH 5.5 to pH 10.0 Thermus thermophilus ?
-
?

Subunits

Subunits Comment Organism
hexamer consisting of three dimers, crystallization data Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.42
-
NADP+ pH 7.4, 50°C Thermus thermophilus
1.6
-
NAD+ pH 7.4, 50°C Thermus thermophilus
1.6
-
1-pyrroline-5-carboxylate pH 7.4, 50°C Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 10 broad Thermus thermophilus