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Literature summary for 1.2.1.87 extracted from

  • Carere, J.; Baker, P.; Seah, S.Y.K.
    Investigating the molecular determinants for substrate channeling in BphI-BphJ, an aldolase-dehydrogenase complex from the polychlorinated biphenyls degradation pathway (2011), Biochemistry, 50, 8407-8416.
    View publication on PubMed
Search for more literature for 1.2.1.87

Cloned(Commentary)

Cloned (Comment) Organism
bphI and bphJ cloned into the plasmids pBTL4-T7 and pET28a Paraburkholderia xenovorans

Protein Variants

Protein Variants Comment Organism
I195F created by site-specific mutagenesis, no significant reduction of channeling efficiency of the enzyme complex toward acetaldehyde or propionaldehyde Paraburkholderia xenovorans
I195L created by site-specific mutagenesis Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 3 propionaldehyde wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
7.4
-
 acetaldehyde I195L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
7.7
-
 propionaldehyde I195L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
24
-
 acetaldehyde wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
35
-
 acetaldehyde I195F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
48
-
 propionaldehyde I195F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans acetyl-CoA + NADH + H+
-
 ?
propionaldehyde + CoA + NAD+
-
 Paraburkholderia xenovorans propanoyl-CoA + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
BphJ
-
 Paraburkholderia xenovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.9
-
 propionaldehyde I195F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
16
-
 acetaldehyde I195F mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
16.3
-
 propionaldehyde wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
17.2
-
 acetaldehyde wild-type protein, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
42
-
 acetaldehyde I195L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans
46
-
 propionaldehyde I195L mutant, steady state, pH 8.0, 25°C Paraburkholderia xenovorans

General Information

General Information Comment Organism
metabolism enzyme is part of the BphI-BphJ-complex, an aldolase-dehydrogenase-complex from the polychlorinated biphenyl degradation pathway Paraburkholderia xenovorans