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Literature summary for 1.2.1.82 extracted from

  • Diaz-Sanchez, V.; Estrada, A.F.; Trautmann, D.; Al-Babili, S.; Avalos, J.
    The gene carD encodes the aldehyde dehydrogenase responsible for neurosporaxanthin biosynthesis in Fusarium fujikuroi (2011), FEBS J., 278, 3164-3176.
    View publication on PubMed
Search for more literature for 1.2.1.82

Cloned(Commentary)

Cloned (Comment) Organism
carD expressed in Escherichia coli BL21 strain Fusarium fujikuroi
generation of an Escherichia coli strain producing beta-apo-4'-carotenal, which is converted into neurosporaxanthin upon expression of carD Fusarium fujikuroi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-apo-4'-carotenal + NAD+ + H2O Fusarium fujikuroi carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal beta-apo-4'-carotenoic acid + NADH + H+ beta-apo-4'-carotenoic acid i.e. neurosporaxanthin ?

Organism

Organism UniProt Comment Textmining
Fusarium fujikuroi F6IBC7
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by generating of a crude lysate for in vitro assays Fusarium fujikuroi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apo-8'-lycopenal + NAD+ + H2O
-
 Fusarium fujikuroi apo-8'-lycopenoic acid + NADH + 2 H+
-
 ?
apo-8'-lycopenal + NAD+ + H2O
-
 Fusarium fujikuroi apo-8'-lycopenoic acid + NADH + H+
-
 ?
beta-apo-10'-carotenal + NAD+ + H2O
-
 Fusarium fujikuroi beta-apo-10'-carotenoic acid + NADH + H+
-
 ?
beta-apo-10'-carotenal + NAD+ + H2O
-
 Fusarium fujikuroi beta-apo-10'-carotenoic acid + NADH + 2 H+
-
 ?
beta-apo-4'-carotenal + NAD+ + H2O carD function substantiated by its targeted disruption in a Fusarium fujikuroi carotenoid-overproducing strain, which resulted in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal Fusarium fujikuroi beta-apo-4'-carotenoic acid + NADH + H+ beta-apo-4'-carotenoic acid i.e. neurosporaxanthin ?
beta-apo-4'-carotenal + NAD+ + H2O
-
 Fusarium fujikuroi neurosporaxanthin + NADH + 2 H+
-
 ?
beta-apo-8'-carotenal + NAD+ + H2O
-
 Fusarium fujikuroi beta-apo-8'-carotenoic acid + NADH + H+
-
 ?
beta-apo-8'-carotenal + NAD+ + H2O
-
 Fusarium fujikuroi beta-apo-8'-carotenoic acid + NADH + 2 H+
-
 ?
additional information no substrate: retinal Fusarium fujikuroi ?
-
 ?
additional information C20-retinal i.e. beta-apo-15'-carotenal is not converted by carD enzymatic activity, indicating the requirement for a minimal length of the substrate chain Fusarium fujikuroi ?
-
 ?

Synonyms

Synonyms Comment Organism
CarD
-
 Fusarium fujikuroi

Expression

Organism Comment Expression
Fusarium fujikuroi CarD mRNA content is enhanced by light, and CarD mRNA levels are higher in carotenoid-overproducing mutants up
Fusarium fujikuroi carD mRNA levels increase about 3fold after 30 min of illumination up

General Information

General Information Comment Organism
physiological function targeted disruption of CarD in a Gibberella fujikuroi carotenoid-overproducing strain results in the loss of neurosporaxanthin and the accumulation of beta-apo-4'-carotenal, its derivative beta-apo-4'-carotenol, and minor amounts of other carotenoids. Strains harboring the CarD mutation acquire a yellowish pigmentation Fusarium fujikuroi