Cloned (Comment) | Organism |
---|---|
expession in Escherichia coli | Nostoc punctiforme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations | Nostoc punctiforme | |
NH4+ | K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations | Nostoc punctiforme | |
Rb+ | K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations | Nostoc punctiforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
stearoyl-[acyl-carrier protein] | presence of potassium ions, pH 7.6, 37°C | Nostoc punctiforme | |
0.027 | - |
stearoyl-[acyl-carrier protein] | pH 7.6, 37°C | Nostoc punctiforme |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations | Nostoc punctiforme | |
NH4+ | K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations | Nostoc punctiforme | |
Rb+ | K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations | Nostoc punctiforme |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | B2J1M0 | - |
- |
Nostoc punctiforme ATCC 29133 | B2J1M0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | codon-optimized gene for expression in Escherichia coli | Nostoc punctiforme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
stearoyl-[acyl-carrier protein] + NAD(P)H + H+ | - |
Nostoc punctiforme | stearylaldehyde + an [acyl-carrier protein] + NAD(P)+ | the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product | ? | |
stearoyl-[acyl-carrier protein] + NAD(P)H + H+ | - |
Nostoc punctiforme ATCC 29133 | stearylaldehyde + an [acyl-carrier protein] + NAD(P)+ | the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product | ? |
Synonyms | Comment | Organism |
---|---|---|
Npun_R1710 | - |
Nostoc punctiforme |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
stearoyl-[acyl-carrier protein] | pH 7.6, 37°C | Nostoc punctiforme | |
0.0028 | - |
stearoyl-[acyl-carrier protein] | presence of potassium ions, pH 7.6, 37°C | Nostoc punctiforme |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Nostoc punctiforme |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme and aldehyde-deformylating oxygenase ADO form a tight isolable complex with a Kd of 3 microM. When the aldehyde substrate is supplied to ADO by acyl-acyl carrier protein reductase, efficient in vitro turnover is observed in the absence of solubilizing agents. Catalysis by acyl-acyl carrier protein reductase proceeds via formation of a covalent intermediate involving residue C294. The enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product | Nostoc punctiforme |