Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Gold cells | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
G234A | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
G234S | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
G234T | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
H448F | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
H448F/P449M | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
H448F/P449M/Y450L | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
H448F/Y450L | the mutant demonstrates a complete loss of substrate inhibition | Staphylococcus aureus |
L161M/Q162M | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
P449M/Y450L | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
Q162M | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
S290T | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
V288D | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
W456H | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
Y450L | the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Betaine aldehyde | substrate inhibition at concentrations of betaine aldehyde as low as 0.15 mM | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
Betaine aldehyde | mutant enzyme G234T, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.12 | - |
Betaine aldehyde | mutant enzyme G234A, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.12 | - |
NAD+ | mutant enzyme G234S, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.17 | - |
Betaine aldehyde | wild type enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.17 | - |
Betaine aldehyde | mutant enzyme G234S, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.26 | - |
NAD+ | wild type enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.26 | - |
Betaine aldehyde | mutant enzyme H448F, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.27 | - |
NAD+ | mutant enzyme V288D, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.3 | - |
NAD+ | mutant enzyme G234T, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.34 | - |
NAD+ | mutant enzyme H448F/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.38 | - |
NAD+ | mutant enzyme W456H, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.41 | - |
NAD+ | mutant enzyme S290T, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.42 | - |
NAD+ | mutant enzyme H448F, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.43 | - |
NAD+ | mutant enzyme P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.57 | - |
Betaine aldehyde | mutant enzyme H448F/P449M, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.68 | - |
NAD+ | mutant enzyme Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.71 | - |
NAD+ | mutant enzyme L161M7Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.91 | - |
Betaine aldehyde | mutant enzyme Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.01 | - |
NAD+ | mutant enzyme Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.12 | - |
NAD+ | mutant enzyme G234A, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.12 | - |
NAD+ | mutant enzyme H448F/P449M, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.18 | - |
Betaine aldehyde | mutant enzyme H448F/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.92 | - |
Betaine aldehyde | mutant enzyme Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.94 | - |
Betaine aldehyde | mutant enzyme P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
2.16 | - |
Betaine aldehyde | mutant enzyme L161M7Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
3.42 | - |
NAD+ | mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
4.61 | - |
Betaine aldehyde | mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
4.76 | - |
Betaine aldehyde | mutant enzyme V288D, at pH 8.0 and 30°C | Staphylococcus aureus | |
13.8 | - |
Betaine aldehyde | mutant enzyme S290T, at pH 8.0 and 30°C | Staphylococcus aureus | |
14.8 | - |
Betaine aldehyde | mutant enzyme W456H, at pH 8.0 and 30°C | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Staphylococcus aureus | - |
betaine + NADH + 2 H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | - |
Staphylococcus aureus | betaine + NADH + 2 H+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
BADH | - |
Staphylococcus aureus |
BetB | - |
Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Betaine aldehyde | mutant enzyme G234T, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.25 | - |
Betaine aldehyde | mutant enzyme V288D, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.94 | - |
Betaine aldehyde | mutant enzyme H448F, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.09 | - |
Betaine aldehyde | mutant enzyme H448F/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.14 | - |
Betaine aldehyde | mutant enzyme G234A, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.35 | - |
Betaine aldehyde | mutant enzyme Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.63 | - |
Betaine aldehyde | mutant enzyme S290T, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.8 | - |
Betaine aldehyde | mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
1.87 | - |
Betaine aldehyde | mutant enzyme P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
2.92 | - |
Betaine aldehyde | mutant enzyme G234S, at pH 8.0 and 30°C | Staphylococcus aureus | |
3.2 | - |
Betaine aldehyde | mutant enzyme W456H, at pH 8.0 and 30°C | Staphylococcus aureus | |
4.06 | - |
Betaine aldehyde | mutant enzyme H448F/P449M, at pH 8.0 and 30°C | Staphylococcus aureus | |
4.46 | - |
Betaine aldehyde | mutant enzyme L161M7Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
9.9 | - |
Betaine aldehyde | mutant enzyme Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
11 | - |
Betaine aldehyde | wild type enzyme, at pH 8.0 and 30°C | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme can use both NAD+ and NADP+ as cofactors, but the NAD+-dependent activity is at least 10 times higher | Staphylococcus aureus | |
NADP+ | the enzyme can use both NAD+ and NADP+ as cofactors, but the NAD+-dependent activity is at least 10 times higher | Staphylococcus aureus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
Betaine aldehyde | pH and temperature not specified in the publication | Staphylococcus aureus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
Betaine aldehyde | mutant enzyme V288D, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.12 | - |
Betaine aldehyde | mutant enzyme S290T, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.22 | - |
Betaine aldehyde | mutant enzyme W456H, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.39 | - |
Betaine aldehyde | mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.7 | - |
Betaine aldehyde | mutant enzyme Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.91 | - |
Betaine aldehyde | mutant enzyme G234T, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.92 | - |
Betaine aldehyde | mutant enzyme H448F/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.96 | - |
Betaine aldehyde | mutant enzyme P449M/Y450L, at pH 8.0 and 30°C | Staphylococcus aureus | |
2.06 | - |
Betaine aldehyde | mutant enzyme L161M7Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
3.62 | - |
Betaine aldehyde | mutant enzyme H448F, at pH 8.0 and 30°C | Staphylococcus aureus | |
7.12 | - |
Betaine aldehyde | mutant enzyme H448F/P449M, at pH 8.0 and 30°C | Staphylococcus aureus | |
9.5 | - |
Betaine aldehyde | mutant enzyme G234A, at pH 8.0 and 30°C | Staphylococcus aureus | |
10.9 | - |
Betaine aldehyde | mutant enzyme Q162M, at pH 8.0 and 30°C | Staphylococcus aureus | |
17.2 | - |
Betaine aldehyde | mutant enzyme G234S, at pH 8.0 and 30°C | Staphylococcus aureus | |
64.7 | - |
Betaine aldehyde | wild type enzyme, at pH 8.0 and 30°C | Staphylococcus aureus |