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Literature summary for 1.2.1.8 extracted from

  • Gonzalez-Segura, L.; Rudino-Pinera, E.; Munoz-Clares, R.A.; Horjales, E.
    The crystal structure of a ternary complex of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa provides new insight into the reaction mechanism and shows a novel binding mode of the 2-phosphate of NADP+ and a novel cation binding site (2009), J. Mol. Biol., 385, 542-557.
    View publication on PubMed
Search for more literature for 1.2.1.8

Application

Application Comment Organism
medicine the enzyme could be a target for antibiotic design Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas aeruginosa
plasmid pCALbetB containing the full sequence of the gene betB that encodes PaBADH is used for the expression of the enzyme in Escherichia coli cells Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
BADH in the presence of 2-mercaptoethanol, glycerol, NADP+ and 150 mM K+, hanging drop vapor diffusion method, using 85 mM HEPES–NaOH, pH 7.5, 8.5% (v/v) isopropanol, 17% (w/v) polyethylene glycol 4000 and 15% (v/v) glycerol, at 18°C Pseudomonas aeruginosa
the three dimensional structure of betaine aldehyde dehydrogenase, in complex with glycerol, NADP+ and K+ ions, is determined at 2.1 A resolution Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Pseudomonas aeruginosa
K+ the enzyme requires K+ ions for stability and possesses two K+ binding sites per subunit Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD(P)+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NAD(P)H + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa Q9HTJ1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa
by a two step procedure Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD(P)+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NAD(P)H + H+
-
 ir
betaine aldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa betaine + NADH + H+
-
 ir
betaine aldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa betaine + NADPH + H+
-
 ir

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase
-
 Pseudomonas aeruginosa
BADH
-
 Pseudomonas aeruginosa
betaine aldehyde dehydrogenase
-
 Pseudomonas aeruginosa
betaine aldehyde: NAD(P)+ oxidoreductase
-
 Pseudomonas aeruginosa
PaBADH
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+ PaBADH uses NADP+ with the same efficiency as NAD+ Pseudomonas aeruginosa
NAD+ BADH uses NAD+ with the same efficiency as NADP+ Pseudomonas aeruginosa
NADP+ PaBADH uses NADP+ with the same efficiency as NAD+ Pseudomonas aeruginosa
NADP+ BADH uses NADP+ with the same efficiency as NAD+ Pseudomonas aeruginosa