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Literature summary for 1.2.1.8 extracted from

  • Nagasawa, T.; Kawabata, Y.; Tani, Y.; Ogata, K.
    Purification and characterization of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa A-16 (1976), Agric. Biol. Chem., 40, 1743-1749.
No PubMed abstract available
Search for more literature for 1.2.1.8

General Stability

General Stability Organism
dialysis for 5 days against 0.1 M buffer, pH 7.4, at 4°C, stable Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline no inhibition at 1 mM Pseudomonas aeruginosa
2,2'-dipyridyl no inhibition at 3 mM Pseudomonas aeruginosa
acetaldehyde 10 mM, 69% inhibition Pseudomonas aeruginosa
Acetylcholine 10 mM, 18% inhibition Pseudomonas aeruginosa
AgNO3 3 mM, 90-100% inhibition Pseudomonas aeruginosa
benzyltrimethylamine iodide 10 mM, 45% inhibition Pseudomonas aeruginosa
betaine 10 mM, 14% inhibition Pseudomonas aeruginosa
choline 10 mM, 23% inhibition Pseudomonas aeruginosa
Glutaraldehyde 10 mM, 83% inhibition Pseudomonas aeruginosa
Hg2+ 3 mM HgCl2, 90-100% inhibition Pseudomonas aeruginosa
iodoacetate 1 mM Pseudomonas aeruginosa
Isobutanal 10 mM, 93% inhibition Pseudomonas aeruginosa
n-butylaldehyde 10 mM, 96% inhibition Pseudomonas aeruginosa
NH4+
-
 Pseudomonas aeruginosa
PCMB 0.01 mM Pseudomonas aeruginosa
tetraethylamine iodide 10 mM, 19% inhibition Pseudomonas aeruginosa
tetramethylamine iodide 10 mM, 19% inhibition Pseudomonas aeruginosa
tetrapropylamine iodide 10 mM, 43% inhibition Pseudomonas aeruginosa
ZnCl2 3 mM, 90-100% inhibition Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.089
-
 NADP+
-
 Pseudomonas aeruginosa
0.22
-
 NAD+
-
 Pseudomonas aeruginosa
0.38
-
 Betaine aldehyde
-
 Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium betaine + NADH
-
 ?
betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa A-16 inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium betaine + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 A-16
-
Pseudomonas aeruginosa A-16
-
 A-16
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Storage Stability

Storage Stability Organism
-20°C, 9.1 M buffer, pH 7.4, 3 weeks, stable Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O reverse reaction not detected Pseudomonas aeruginosa betaine + NADH
-
 ir
betaine aldehyde + NAD+ + H2O inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium Pseudomonas aeruginosa betaine + NADH
-
 ?
betaine aldehyde + NAD+ + H2O reverse reaction not detected Pseudomonas aeruginosa A-16 betaine + NADH
-
 ir
betaine aldehyde + NAD+ + H2O inducible enzyme accumulates in the presence of choline, acetylcholine or betaine in the medium Pseudomonas aeruginosa A-16 betaine + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 7.4, 0.01% 2-mercaptoethanol, 7 min, 50% loss of activity Pseudomonas aeruginosa
50
-
 pH 7.4, 0.01% 2-mercaptoethanol, 7 min, complete loss of activity Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 25% of maximal activity, pH 9.5: about 50% of maximal activity Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 15 h, 80% loss of activity Pseudomonas aeruginosa
5 7.5 15 h, stable Pseudomonas aeruginosa
8
-
 15 h, 60% loss of activity Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas aeruginosa
NADP+
-
 Pseudomonas aeruginosa