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Literature summary for 1.2.1.8 extracted from

  • Mori, N.; Kawakami, B.; Hyakutome, K.; Tani, Y.; Yamada, H.
    Characterization of betaine aldehyde dehydrogenase from Cylindrocarpon didynum M-1 (1980), Agric. Biol. Chem., 44, 3015-3016.
No PubMed abstract available
Search for more literature for 1.2.1.8

General Stability

General Stability Organism
complete loss of activity when dialyzed against 10 mM buffer, pH 8.0, addition of 20% glycerol stabilizes Cylindrocarpon didymum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Cylindrocarpon didymum
2,2'-dipyridyl
-
 Cylindrocarpon didymum
Co2+
-
 Cylindrocarpon didymum
Cu2+
-
 Cylindrocarpon didymum
Hg2+
-
 Cylindrocarpon didymum
iodoacetate
-
 Cylindrocarpon didymum
PCMB
-
 Cylindrocarpon didymum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 Betaine aldehyde
-
 Cylindrocarpon didymum
0.38
-
 NAD+
-
 Cylindrocarpon didymum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 4 * 58000, SDS-PAGE Cylindrocarpon didymum
220000
-
 gel filtration Cylindrocarpon didymum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Cylindrocarpon didymum synthesis of betaine betaine + NADH
-
 ?
betaine aldehyde + NAD+ + H2O Cylindrocarpon didymum M-1 synthesis of betaine betaine + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Cylindrocarpon didymum
-
 M-1
-
Cylindrocarpon didymum M-1
-
 M-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cylindrocarpon didymum

Reaction

Reaction Comment Organism Reaction ID
betaine aldehyde + NAD+ + H2O = betaine + NADH + 2 H+ ping-pong mechanism Cylindrocarpon didymum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.5
-
-
 Cylindrocarpon didymum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O
-
 Cylindrocarpon didymum betaine + NADH
-
 ?
betaine aldehyde + NAD+ + H2O synthesis of betaine Cylindrocarpon didymum betaine + NADH
-
 ?
betaine aldehyde + NAD+ + H2O
-
 Cylindrocarpon didymum M-1 betaine + NADH
-
 ?
betaine aldehyde + NAD+ + H2O synthesis of betaine Cylindrocarpon didymum M-1 betaine + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 58000, SDS-PAGE Cylindrocarpon didymum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
 Cylindrocarpon didymum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Cylindrocarpon didymum