Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | about 35% activation at 5 mM | Synechocystis sp. PCC 6803 | |
citrate | about 25% activation at 5 mM | Synechocystis sp. PCC 6803 | |
oxaloacetate | about 15% activation at 5 mM | Synechocystis sp. PCC 6803 |
Cloned (Comment) | Organism |
---|---|
gene gabD, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha | Synechocystis sp. PCC 6803 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by citrate | Synechocystis sp. PCC 6803 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Synechocystis sp. PCC 6803 | |
0.01 | - |
succinate semialdehyde | pH 9.7, 45°C, recombinant detagged enzyme | Synechocystis sp. PCC 6803 | |
0.058 | - |
NADP+ | pH 9.7, 45°C, recombinant detagged enzyme | Synechocystis sp. PCC 6803 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Synechocystis sp. PCC 6803 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate semialdehyde + NADP+ + H2O | Synechocystis sp. PCC 6803 | - |
succinate + NADPH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | Q55585 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain DH5alpha by glutathione affinity chromatography and ultrafiltration, followed by tag cleavage through PreScission protease in a batch and the protease resin is eliminated by centrifugation | Synechocystis sp. PCC 6803 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate semialdehyde + NADP+ + H2O | - |
Synechocystis sp. PCC 6803 | succinate + NADPH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gabD | - |
Synechocystis sp. PCC 6803 |
slr0370 | locus name | Synechocystis sp. PCC 6803 |
SSADH | - |
Synechocystis sp. PCC 6803 |
succinic semialdehyde dehydrogenase | - |
Synechocystis sp. PCC 6803 |
SySSADH | - |
Synechocystis sp. PCC 6803 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Synechocystis sp. PCC 6803 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.38 | - |
succinate semialdehyde | pH 9.7, 45°C, recombinant detagged enzyme | Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.7 | - |
recombinant enzyme | Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Synechocystis sp. PCC 6803 |
General Information | Comment | Organism |
---|---|---|
metabolism | SySSADH catalyzes one of the NAD(P)H generating reactions in the oxidative TCA cycle. The oxidative TCA cycle is a pathway with low efficiency in NADPH generation in Synechocystis sp. 6803. Similar to isocitrate dehydrogenase from Synechocystis sp. 6803, SySSADH specifically catalyzes the NADPH-generating reaction and is not inhibited by citrate. To avoid NADPH overproduction, the oxidative pentose phosphate (OPP) pathway dehydrogenase activity is repressed when the flow of the oxidative TCA cycle increases in Synechocystis sp. 6803. Metabolic map around the OPP pathway, overview | Synechocystis sp. PCC 6803 |
physiological function | succinic semialdehyde dehydrogenase (SSADH) from Synechocystis 6803 (SySSADH) catalyzes one of the NAD(P)H generating reactions in the oxidative TCA cycle. Similar to isocitrate dehydrogenase from Synechocystis sp. 6803, SySSADH specifically catalyzes the NADPH-generating reaction and is not inhibited by citrate. The activity of SySSADH is lower than that of other bacterial SSADHs | Synechocystis sp. PCC 6803 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.8 | - |
NADP+ | pH 9.7, 45°C, recombinant detagged enzyme | Synechocystis sp. PCC 6803 | |
138 | - |
succinate semialdehyde | pH 9.7, 45°C, recombinant detagged enzyme | Synechocystis sp. PCC 6803 |