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Literature summary for 1.2.1.79 extracted from

  • Jaeger, M.; Rothacker, B.; Ilg, T.
    Saturation transfer difference NMR studies on substrates and inhibitors of succinic semialdehyde dehydrogenases (2008), Biochem. Biophys. Res. Commun., 372, 400-406.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3-tolualdehyde only the aldehyde forms and not the gem-diol forms of the inhibitor 3-tolualdehyde bind to the enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
succinate semialdehyde pH 8.5, 22°C Escherichia coli
0.0446
-
NADP+ pH 8.5, 22°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P25526
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34
-
pH 8.5, 22°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-carboxybenzaldehyde + NADP+ + H2O
-
Escherichia coli 3-carboxybenzoate + NADPH + 2 H+
-
?
3-nitrobenzaldehyde + NADP+ + H2O
-
Escherichia coli 3-nitrobenzoate + NADPH + 2 H+
-
?
4-carboxybenzaldehyde + NADP+ + H2O
-
Escherichia coli 4-carboxybenzoate + NADPH + H+
-
?
benzaldehyde + NADP+ + H2O
-
Escherichia coli benzoate + NADPH + 2 H+
-
?
additional information only the aldehyde forms and not the gem-diol forms of the specific substrate succinic semialdehyde , of selected aldehyde substrates, and of the inhibitor 3-tolualdehyde bind to the enzyme Escherichia coli ?
-
?
n-butanal + NADP+ + H2O
-
Escherichia coli butanoate + NADPH + 2 H+
-
?
n-hexanal + NADP+ + H2O
-
Escherichia coli hexanoate + NADPH + H+
-
?
n-pentanal + NADP+ + H2O
-
Escherichia coli n-pentanoate + NADPH + H+
-
?
succinate semialdehyde + NADP+ + H2O
-
Escherichia coli succinate + NADPH + 2 H+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.4
-
75% of maximum activity Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ Escherichia coli
NADP+ NAD+ also acts as cosubstrate, but the reaction rates are more than 20fold lower than those with NADP+ Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.08
-
pH 8.5, 22°C Escherichia coli 3-tolualdehyde