Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.69 extracted from

  • Murphy, C.D.; Moss, S.J.; O'Hagan, D.
    Isolation of an aldehyde dehydrogenase involved in the oxidation of fluoroacetaldehyde to fluoroacetate in Streptomyces cattleya (2001), Appl. Environ. Microbiol., 67, 4919-4921.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.69

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, increase in activity Streptomyces cattleya

Inhibitors

Inhibitors Comment Organism Structure
Chloroacetaldehyde 0.25 mM, time-dependent inactivation Streptomyces cattleya
Cu2+ 1 mM, strong inhibition Streptomyces cattleya
iodoacetamide 0.05 mM, strong inhibition Streptomyces cattleya
Mg2+ 1 mM, 80% loss of activity, possibly due to a decreased dissociation rate of NADH from the enzyme Streptomyces cattleya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 fluoroacetaldehyde
-
 Streptomyces cattleya
0.15
-
 glycoaldehyde
-
 Streptomyces cattleya
0.32
-
 benzaldehyde
-
 Streptomyces cattleya
0.81
-
 acetaldehyde
-
 Streptomyces cattleya

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 4 * 55000, SDS-PAGE Streptomyces cattleya
200000
-
 gel filtration Streptomyces cattleya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fluroracetaldehyde + NAD+ + H2O Streptomyces cattleya involved in fluorometabolite biosynthesis fluoroacetate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces cattleya
-
 NRRL 8057
-
Streptomyces cattleya NRRL 8057
-
 NRRL 8057
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, anion-exchange, 5'-AMP-agarose Streptomyces cattleya

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.889
-
-
 Streptomyces cattleya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O 40% of activity with fluoroacetaldehyde Streptomyces cattleya acetate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O 40% of activity with fluoroacetaldehyde Streptomyces cattleya NRRL 8057 acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O 65% of activity with fluoroacetaldehyde Streptomyces cattleya benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O 65% of activity with fluoroacetaldehyde Streptomyces cattleya NRRL 8057 benzoate + NADH + H+
-
 ?
chloroacetaldehyde + NAD+ + H2O 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation Streptomyces cattleya chloroacetate + NADH + H+
-
 ?
chloroacetaldehyde + NAD+ + H2O 80% of activity with fluoroacetaldehyde, inactivation upon extended incubation Streptomyces cattleya NRRL 8057 chloroacetate + NADH + H+
-
 ?
DL-glyceraldehyde + NAD+ + H2O 10% of activity with fluoroacetaldehyde Streptomyces cattleya DL-glycerate + NADH + H+
-
 ?
fluroracetaldehyde + NAD+ + H2O
-
 Streptomyces cattleya fluoroacetate + NADH + H+
-
 ?
fluroracetaldehyde + NAD+ + H2O
-
 Streptomyces cattleya NRRL 8057 fluoroacetate + NADH + H+
-
 ?
fluroracetaldehyde + NAD+ + H2O involved in fluorometabolite biosynthesis Streptomyces cattleya fluoroacetate + NADH
-
 ?
glycoaldehyde + NAD+ + H2O 150% of activity with fluoroacetaldehyde Streptomyces cattleya glycolate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O 28% of activity with fluoroacetaldehyde Streptomyces cattleya propionate + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O 28% of activity with fluoroacetaldehyde Streptomyces cattleya NRRL 8057 propionate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Streptomyces cattleya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Streptomyces cattleya