Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | maximum 12% increase of activity observed with 0.1 mM | Daucus carota | |
additional information | the activity is not strictly dependent on the presence of a reducing agent, such as DTT in the reaction mixture | Daucus carota |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0548 | - |
4-hydroxybenzaldehyde | apparent Km-value, pH 7.5 and 35°C | Daucus carota | |
0.0744 | - |
NAD+ | apparent Km-value, pH 7.5 and 35°C | Daucus carota |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM chloride salt, 87.2% relative activity compared to control | Daucus carota | |
Co2+ | 1 mM chloride salt, 67.5% relative activity compared to control | Daucus carota | |
Cu2+ | 1 mM chloride salt, 34.7% relative activity compared to control | Daucus carota | |
Fe2+ | 1 mM chloride salt, 93.6% relative activity compared to control | Daucus carota | |
Mg2+ | 1 mM chloride salt, 88% relative activity compared to control | Daucus carota | |
Mn2+ | 1 mM chloride salt, 80.6% relative activity compared to control | Daucus carota | |
Zn2+ | 1 mM chloride salt, 49.9% relative activity compared to control | Daucus carota |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
hairy root cultures | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0072 | - |
constant activity in non-treated root lines, pH 7.5 and 35°C | Daucus carota |
0.0072 | - |
up to 6 h of methyl jasmonate treatment, pH 7.5 and 35°C | Daucus carota |
0.0384 | - |
peak after 24 h of methyl jasmonate treatment, pH 7.5 and 35°C | Daucus carota |
Storage Stability | Organism |
---|---|
a loss of 4-hydroxybenzaldehyde dehydrogenase activity to the range of 75%, 35% and 20% of the optimum value is observed when cell-free extracts are frozen in liquid nitrogen and stored at +4, -20 and -80°C, for 24 h, respectively | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxybenzaldehyde + NAD+ + H2O | 12.3% relative activity compared to 4-hydroxybenzaldehyde conversion | Daucus carota | 2-hydroxybenzoate + NADH + 2 H+ | - |
? | |
2-methoxybenzaldehyde + NAD+ + H2O | 4.8% relative activity compared to 4-hydroxybenzaldehyde conversion, 2-methoxybenzaldehyde i.e. o-anisaldehyde | Daucus carota | 2-methoxybenzoate + NADH + 2 H+ | 2-methoxybenzoate i.e. o-anisic acid | ? | |
3-hydroxybenzaldehyde + NAD+ + H2O | 27.4% relative activity compared to 4-hydroxybenzaldehyde conversion | Daucus carota | 3-hydroxybenzoate + NADH + 2 H+ | - |
? | |
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O | 1% relative activity compared to 4-hydroxybenzaldehyde conversion, 4-hydroxy-3-methoxybenzaldehyde i.e. vanillin | Daucus carota | 4-hydroxy-3-methoxybenzoate + NADH + 2 H+ | 4-hydroxy-3-methoxybenzoate i.e. vanillic acid | ? | |
4-hydroxybenzaldehyde + FAD + H2O | 17.6% relative activity compared to reaction with NAD+ | Daucus carota | 4-hydroxybenzoate + FADH2 + ? | - |
? | |
4-hydroxybenzaldehyde + FMN + H2O | 11.5% relative activity compared to reaction with NAD+ | Daucus carota | 4-hydroxybenzoate + FMNH2 + ? | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | preferred substrate, rapid increase in 4-hydroxybenzoic acid accumulation in Daucus carota hairy root cultures detectable between 15 and 25 days after subculture preceded by an increase in 4-hydroxybenzaldehyde dehydrogenase activity | Daucus carota | 4-hydroxybenzoate + NADH + 2 H+ | - |
ir | |
4-hydroxybenzaldehyde + NADP+ + H2O | 70.4% relative activity compared to reaction with NAD+ | Daucus carota | 4-hydroxybenzoate + NADPH + 2 H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 5.8% relative activity compared to 4-hydroxybenzaldehyde conversion | Daucus carota | benzoate + NADH + 2 H+ | - |
? | |
cinnamaldehyde + NAD+ + H2O | 6.13% relative activity compared to 4-hydroxybenzaldehyde conversion | Daucus carota | cinnamic acid + NADH + 2 H+ | - |
? | |
additional information | no activity with 3,4-dihydroxybenzaldehyde i.e. protocatechuic aldehyde and 3,4,5-trihydroxybenzaldehyde | Daucus carota | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxybenzaldehyde dehydrogenase | - |
Daucus carota |
HBD | - |
Daucus carota |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Daucus carota |
Organism | Comment | Expression |
---|---|---|
Daucus carota | hairy root cultures of Daucus carota respond to methyl jasmonate treatment with enhanced accumulation of 4-hydroxybenzoic acid | up |
General Information | Comment | Organism |
---|---|---|
malfunction | 4-hydroxybenzaldehyde dehydrogenase is the final enzyme of 4-hydroxybenzoic acid biosynthesis | Daucus carota |