Inhibitors | Comment | Organism | Structure |
---|---|---|---|
m-hydroxybenzaldehyde | - |
Escherichia coli | |
NADH | - |
Escherichia coli | |
p-Hydroxybenzaldehyde | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
5-carboxymethyl-2-hydroxymuconate semialdehyde | - |
Escherichia coli | |
0.052 | - |
NAD+ | - |
Escherichia coli | |
3.6 | - |
NADP+ | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
4 * 58000, SDS-PAGE | Escherichia coli |
210000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Escherichia coli | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
54 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Escherichia coli | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NADP+ | 11% of the activity compared to the reduction with NAD+ | Escherichia coli | 5-carboxymethyl-2-hydroxymuconate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 58000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
less active in Tris-HCl buffer than in phosphate or glycine/NaOH buffers | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strict dependency for NAD+ reduction | Escherichia coli | |
NADP+ | 89% lower activity compared to NAD+ | Escherichia coli |