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Literature summary for 1.2.1.59 extracted from

  • Tourigny, D.S.; Elliott, P.R.; Edgell, L.J.; Hudson, G.M.; Moody, P.C.
    Expression, purification, crystallization and preliminary X-ray analysis of wild-type and of an active-site mutant of glyceraldehyde-3-phosphate dehydrogenase from Campylobacter jejuni (2011), Acta Crystallogr. Sect. F, 67, 72-75.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.59

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta DE3 cells Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type and mutant enzyme C150S, sitting drop vapor diffusion method, using 25% (w/v) PEG 1K, 175 mM sodium/potassium tartrate and 100 mM sodium acetate pH 4.5 Campylobacter jejuni

Protein Variants

Protein Variants Comment Organism
C150S the mutant is unable to turn over D-glyceraldehyde 3-phosphate in the presence of either NAD+ or NADP+ Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Campylobacter jejuni
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NAD+ Campylobacter jejuni NCTC 11168
-
 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Campylobacter jejuni
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NADP+ Campylobacter jejuni NCTC 11168
-
 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Campylobacter jejuni NCTC 11168
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap nickel Sepharose column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Campylobacter jejuni 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Campylobacter jejuni NCTC 11168 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Campylobacter jejuni 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r
D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Campylobacter jejuni NCTC 11168 3-phospho-D-glyceroyl phosphate + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
cgap
-
 Campylobacter jejuni
GAPDH
-
 Campylobacter jejuni

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme can utilize either NADP+ or NAD+ as coenzymes Campylobacter jejuni
NADP+ the enzyme can utilize either NADP+ or NAD+ as coenzymes Campylobacter jejuni