Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not arsenite | Euglena gracilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
CoA | - |
Euglena gracilis | |
0.03 | - |
NADP+ | - |
Euglena gracilis | |
0.21 | - |
pyruvate | - |
Euglena gracilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 4 mol per mol subunit | Euglena gracilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
166000 | - |
2 * 166000, SDS-PAGE | Euglena gracilis |
309000 | - |
gel filtration | Euglena gracilis |
309000 | - |
I. methyl viologen-pyruvate dehydrogenase fragment with iron-sulfur cluster | Euglena gracilis |
309000 | - |
two covalently linked domains | Euglena gracilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + NADP+ | Euglena gracilis | - |
acetyl-CoA + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis | - |
- |
- |
Oxidation Stability | Organism |
---|---|
O2 leads to rapid inactivation | Euglena gracilis |
Purification (Comment) | Organism |
---|---|
- |
Euglena gracilis |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.93 | - |
3-hydroxypyruvate | Euglena gracilis |
2.36 | - |
2-oxobutyrate | Euglena gracilis |
2.6 | - |
oxaloacetate | Euglena gracilis |
14.3 | - |
pyruvate | Euglena gracilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + CoA + NADP+ | - |
Euglena gracilis | propionyl-CoA + CO2 + NADPH | - |
? | |
3-hydroxypyruvate + CoA + NADP+ | - |
Euglena gracilis | hydoxyacetyl-CoA + CO2 + NADPH | - |
? | |
oxaloacetate + CoA + NADP+ | - |
Euglena gracilis | ? + CO2 + NADPH | - |
? | |
pyruvate + CoA + NADP+ | - |
Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r | |
pyruvate + CoA + NADP+ | NADP+ can be substituted by methyl viologen | Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r | |
pyruvate + CoA + NADP+ | NADP+ can be substituted by benzyl viologen | Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r | |
pyruvate + CoA + NADP+ | reverse reaction 2% of forward reaction | Euglena gracilis | acetyl-CoA + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 166000, SDS-PAGE | Euglena gracilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Euglena gracilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Euglena gracilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Euglena gracilis | |
NADP+ | not NAD+ | Euglena gracilis | |
thiamine diphosphate | - |
Euglena gracilis |