General Stability | Organism |
---|---|
requires thiol reducing agents for stability and activity | Photobacterium phosphoreum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
tetradecanoyl-CoA | - |
Photobacterium phosphoreum | |
0.005 | - |
NADPH | - |
Photobacterium phosphoreum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
50000 | - |
x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
58000 | - |
x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetradecanoyl-CoA + NADPH + H+ | Photobacterium phosphoreum | - |
tetradecanal + CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photobacterium phosphoreum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Photobacterium phosphoreum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Photobacterium phosphoreum |
Storage Stability | Organism |
---|---|
-20°C, 50 mM phosphate buffer, pH 7.0, 0.2 M NaCl, 50 mM beta-mercaptoethanol, 30% glycerol, several months | Photobacterium phosphoreum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tetradecanoyl-CoA + NADPH + H+ | - |
Photobacterium phosphoreum | tetradecanal + CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | x * 58000 + x * 34000 + x * 50000, reductase, transferase and synthetase subunit of the enzyme complex, SDS-PAGE | Photobacterium phosphoreum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | 3% of NADPH activity | Photobacterium phosphoreum | |
NADPH | - |
Photobacterium phosphoreum |