Inhibitors | Comment | Organism | Structure |
---|---|---|---|
barbiturates | - |
Ovis aries | |
glycine buffer | complete blockage of the reaction | Ovis aries | |
hydrazine | 1-3 mM | Ovis aries | |
N-ethylmaleimide | 1-3 mM | Ovis aries | |
N-tris(hydroxymethyl)methylglycine | - |
Ovis aries | |
p-hydroxymercuribenzoate | 1-3 mM | Ovis aries | |
Sodium bisulfite | 1 mM | Ovis aries | |
Sodium cyanide | 1 mM | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
NADP+ | - |
Ovis aries | |
0.21 | - |
methylglyoxal | - |
Ovis aries | |
0.21 | 0.83 | methylglyoxal | - |
Ovis aries |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylglyoxal + NADP+ + H2O | Ovis aries | ir | pyruvate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using acetone powder extract, ammonium sulfate fractionation, alumina C-gamma gel and column chromatography on Sephadex G-200 | Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Ovis aries | - |
liver | - |
Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
- |
Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoaldehydes + NADP+ + H2O | enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23 | Ovis aries | 2-oxo acids + NADPH | - |
? | |
methylglyoxal + NADP+ + H2O | ir | Ovis aries | pyruvate + NADPH + H+ | - |
? |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
not active below | Ovis aries |
9.4 | - |
activity increases up to, Tris buffer | Ovis aries |
10.5 | - |
activity increases up to, carbonate buffer | Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 3-acetyl-pyridine adenine dinucleotide and thionicotinamide adenine dinucleotide are also effective oxidants | Ovis aries | |
NAD+ | enzyme from sheep or rat liver, see also EC 1.2.1.23 | Ovis aries | |
NADP+ | - |
Ovis aries |