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Literature summary for 1.2.1.49 extracted from

  • Dunkerton, J.; James, S.P.
    Purification of 2-oxoaldehyde dehydrogenase and its dependence on unusual amines (1975), Biochem. J., 149, 609-617.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.49

Activating Compound

Activating Compound Comment Organism Structure
2-amino-2-methylpropan-1-ol activation of aldehyde oxidation Ovis aries
2-amino-2-methylpropane-1,3-diol activation of aldehyde oxidation Ovis aries
2-Aminobutan-1-ol activation of aldehyde oxidation Ovis aries
2-aminopropane activation of aldehyde oxidation Ovis aries
2-aminopropane-1,3-diol activation of aldehyde oxidation Ovis aries
L-2-Aminopropan-1-ol activation of aldehyde oxidation Ovis aries
Tris activation of aldehyde oxidation Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
barbiturates
-
 Ovis aries
Nitro-alcohols
-
 Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylglyoxal + NADP+ + H2O Ovis aries
-
 pyruvate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, phosphocellulose, Sephadex G-200, hydroxylapatite, and DEAE-cellulose Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Storage Stability

Storage Stability Organism
4°C, pH 7.4, 50 mM NaCl, 5 mM 2-amino-2-methylpropane-1,3-diol, 20 days Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoaldehydes + NADP+ + H2O enzyme from sheep or rat liver also active with NAD+, see also EC 1.2.1.23 Ovis aries 2-oxo acids + NADPH
-
 ?
methylglyoxal + NADP+ + H2O
-
 Ovis aries pyruvate + NADPH + H+
-
 ?
additional information the presence of L-2-aminopropan-1-ol is necessary to activate the enzyme Ovis aries ?
-
 ?
additional information the presence of amines like Tris, 2-amino-2-methylpropane-1,3-diol, 2-amino-2-methylpropan-1-ol, 2-aminopropane-1,3-diol, 2-aminopropane or 2-aminobutan-1-ol is necessary to activate the enzyme Ovis aries ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
-
 Ovis aries

pH Range

pH Minimum pH Maximum Comment Organism
7.8
-
 not active below Ovis aries

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme from sheep or rat liver, see also EC 1.2.1.23 Ovis aries
NADP+
-
 Ovis aries