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Literature summary for 1.2.1.48 extracted from

  • Kato, T.; Miyanaga, A.; Kanaya, S.; Morikawa, M.
    Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23 (2009), Extremophiles, 14, 33-39.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alkane transcriptional induction of Bt-aldh gene by alkane, Bt-aldh dramatically increases after 10 day cultivation, exactly when the alkane degradation starts Geobacillus thermoleovorans

Cloned(Commentary)

Cloned (Comment) Organism
expression and overproduction in Escherichia coli Geobacillus thermoleovorans
expression in Escherichia coli Geobacillus thermoleovorans

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, 63% residual activity; 63% relative activity Geobacillus thermoleovorans
Mg2+ 14% relative activity with 2 mM MgCl2 compared to the activity without metal ions; 2 mM, 14% residual activity Geobacillus thermoleovorans
Zn2+ 2 mM, 0.1% residual activity; relative activity is above 1% with 2 mM ZnCl2 compared to the activity without metal ions Geobacillus thermoleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activity is elevated 2fold in the presence of 2 mM Geobacillus thermoleovorans
Ba2+ 2 mM, 2-3fold stimulation Geobacillus thermoleovorans
Ca2+ activity is elevated 2fold in the presence of 2 mM Geobacillus thermoleovorans
Ca2+ 2 mM, 2-3fold stimulation Geobacillus thermoleovorans
Sr2+ activity is elevated 3fold in the presence of 2 mM Geobacillus thermoleovorans
Sr2+ 2 mM, 2-3fold stimulation Geobacillus thermoleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53886
-
8 * 53886, calculated, 8 * 55000, SDS-PAGE Geobacillus thermoleovorans
53890
-
monomer is calculated from the 497 amino acid residues Geobacillus thermoleovorans
55000
-
8 * 55000, determined by SDS-PAGE Geobacillus thermoleovorans
55000
-
8 * 53886, calculated, 8 * 55000, SDS-PAGE Geobacillus thermoleovorans
410000
-
gel filtration Geobacillus thermoleovorans
410000
-
estimated by gel filtration Geobacillus thermoleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetaldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
acetate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O Geobacillus thermoleovorans B23
-
acetate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
benzoate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O Geobacillus thermoleovorans B23
-
benzoate + NADH + H+
-
?
betaine aldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
?
-
?
glyceraldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
?
-
?
hexanal + NAD+ + H2O Geobacillus thermoleovorans
-
?
-
?
additional information Geobacillus thermoleovorans enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes ?
-
?
additional information Geobacillus thermoleovorans B23 enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes ?
-
?
octanal + NAD+ + H2O Geobacillus thermoleovorans
-
octanoate + NADH + H+
-
?
octanal + NAD+ + H2O Geobacillus thermoleovorans B23
-
octanoate + NADH + H+
-
?
p-cumic aldehyde + NAD+ + H2O Geobacillus thermoleovorans
-
?
-
?
retinal + NAD+ + H2O Geobacillus thermoleovorans
-
?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus thermoleovorans Q9FAB1
-
-
Geobacillus thermoleovorans B23 Q9FAB1
-
-

Purification (Commentary)

Purification (Comment) Organism
crude enzyme solution is applied to an anionic exchange column and eluted by linearly increasing the NaCl concentration from 0-1.0 M in 50 mM Tris-HCl at pH 7.0 Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
110% relative activity with 2 mM MnCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
120% relative activity with 2 mM CoCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
14% relative activity with 2 mM MgCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
190% relative activity with 2 mM CaCl2 EDTA compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
210% relative activity with 2 mM BaCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
340% relative activity with 2 mM SrCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
63% relative activity with 2 mM EDTA compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
87% relative activity with 2 mM CuCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
97% relative activity with 2 mM NiCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans
additional information
-
in the range of substrate aldehydes with carbon numbers C2 to C14, Bt-Aldh is the most active against C14, tetradecanal at 55°C, the activity against tetradecanal is 73times higher than that against octanal Geobacillus thermoleovorans
additional information
-
relative activity is above 1% with 2 mM ZnCl2 compared to the activity without metal ions, pH 10, 50 mM glycine buffer, 37°C, 1 mM hexanal, 1 mM NAD+ Geobacillus thermoleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Geobacillus thermoleovorans acetate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O
-
Geobacillus thermoleovorans B23 acetate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O
-
Geobacillus thermoleovorans benzoate + NADH + H+
-
?
benzaldehyde + NAD+ + H2O
-
Geobacillus thermoleovorans B23 benzoate + NADH + H+
-
?
betaine aldehyde + NAD+ + H2O
-
Geobacillus thermoleovorans ?
-
?
dodecanal + NAD+ + H2O 50% of the activity with tetradecanal Geobacillus thermoleovorans dodecanoic acid + NADH + H+
-
?
glyceraldehyde + NAD+ + H2O
-
Geobacillus thermoleovorans ?
-
?
hexanal + NAD+ + H2O
-
Geobacillus thermoleovorans ?
-
?
hexanal + NAD+ + H2O 50% of the activity with tetradecanal Geobacillus thermoleovorans hexanoic acid + NADH + H+
-
?
additional information enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes Geobacillus thermoleovorans ?
-
?
additional information enzyme displays high activity against aliphatic long-chain aldehydes such as tetradecanal Geobacillus thermoleovorans ?
-
?
additional information enzyme has high activity against aliphatic long-chain aldehydes such as tetradecanal, Bt-Aldh represents thermophilic aldehyde dehydrogenases responsible for degradation of long-chain alkanes Geobacillus thermoleovorans B23 ?
-
?
additional information enzyme displays high activity against aliphatic long-chain aldehydes such as tetradecanal Geobacillus thermoleovorans B23 ?
-
?
octanal + NAD+ + H2O
-
Geobacillus thermoleovorans octanoate + NADH + H+
-
?
octanal + NAD+ + H2O
-
Geobacillus thermoleovorans B23 octanoate + NADH + H+
-
?
octanal + NAD+ + H2O 50% of the activity with tetradecanal Geobacillus thermoleovorans octanoic acid + NADH + H+
-
?
p-cumic aldehyde + NAD+ + H2O
-
Geobacillus thermoleovorans ?
-
?
retinal + NAD+ + H2O
-
Geobacillus thermoleovorans ?
-
?
tetradecanal + NAD+ + H2O
-
Geobacillus thermoleovorans tetradecanoic acid + NADH + H+
-
?

Subunits

Subunits Comment Organism
octamer 8 * 55000, determined by SDS-PAGE Geobacillus thermoleovorans
octamer 8 * 53886, calculated, 8 * 55000, SDS-PAGE Geobacillus thermoleovorans

Synonyms

Synonyms Comment Organism
Bt-Aldh
-
Geobacillus thermoleovorans
long-chain-aldehyde dehydrogenase
-
Geobacillus thermoleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
Geobacillus thermoleovorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 55 optimum condition for activity Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
stable up to Geobacillus thermoleovorans
70
-
activity decreases to 12.7% after a heat treatment at 70°C for 30 min Geobacillus thermoleovorans
70
-
30 min, 12.7% residual activity Geobacillus thermoleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Geobacillus thermoleovorans

Cofactor

Cofactor Comment Organism Structure
NAD+ activity of Bt-Aldh is doubled in the presence of NAD+ than NADP+ Geobacillus thermoleovorans
NADH
-
Geobacillus thermoleovorans
NADP+
-
Geobacillus thermoleovorans
NADPH about 50% of the activity with NADH Geobacillus thermoleovorans

Expression

Organism Comment Expression
Geobacillus thermoleovorans expression level is dramatically increased when alkane degradation is started up