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Literature summary for 1.2.1.47 extracted from

  • Hassan, M.; Okada, M.; Ichiyanagi, T.; Mori, N.
    4-N-trimethylaminobutyraldehyde dehydrogenase: purification and characterization of an enzyme from Pseudomonas sp. 13CM (2008), Biosci. Biotechnol. Biochem., 72, 155-162.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM causes 5% inhibition at 30°C Pseudomonas sp.
3-methylbutyraldehyde 1 mM causes 57% inhibition at 30°C Pseudomonas sp.
4-N-trimethylaminobutyraldehyde the substrate inhibits at 0.24 mM Pseudomonas sp.
5,5'-dithiobis-(2-nitrobenzoic acid) 0.1 mM causes 100% inhibition at 30°C Pseudomonas sp.
acetaldehyde 1 mM causes 18% inhibition at 30°C Pseudomonas sp.
Ba2+ 1 mM causes 30% inhibition at 30°C Pseudomonas sp.
Betaine aldehyde 1 mM causes 41% inhibition at 30°C Pseudomonas sp.
Co2+ 1 mM causes 18% inhibition at 30°C Pseudomonas sp.
Cu2+ 0.05 mM causes 36% inhibition at 30°C Pseudomonas sp.
diethylmethylamine at 30°C, 5 mM causes 53% inhibition, does not inhibit at 1 mM Pseudomonas sp.
dimethylethylamine 5 mM causes 9% inhibition at 30°C Pseudomonas sp.
ethylmethylamine 1 mM causes 20% inhibition at 30°C Pseudomonas sp.
glyceraldehyde 1 mM causes 7% inhibition at 30°C Pseudomonas sp.
Hg2+ 0.05 mM causes 28% inhibition at 30°C Pseudomonas sp.
iodoacetoamide 1 mM causes 42% inhibition at 30°C Pseudomonas sp.
KCl 1 mM causes 5% inhibition at 30°C Pseudomonas sp.
Li+ 1 mM causes 13% inhibition at 30°C Pseudomonas sp.
additional information at 30°C, not inhibited by Rb+, Na+, Mg2+, Ca2+, potassium fluoride, 1 mM sodium azide, 2,2'-dipyridyl, EDTA, 1 mM trimethylamine and 1 mM tetramethylammonium chloride Pseudomonas sp.
p-chloromercuribenzoate 0.1 mM causes 88% inhibition at 30°C Pseudomonas sp.
phenylacetaldehyde 0.1 mM causes 54% inhibition at 30°C Pseudomonas sp.
Semicarbazide 1 mM causes 22% inhibition at 30°C Pseudomonas sp.
triethylamine 5 mM causes 42% inhibition at 30°C Pseudomonas sp.
trimethylacetaldehyde 1 mM causes 80% inhibition at 30°C Pseudomonas sp.
Zn2+ 1 mM causes 11% inhibition at 30°C Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0074
-
4-N-trimethylaminobutyraldehyde
-
Pseudomonas sp.
0.051
-
4-dimethylaminobutyraldehyde
-
Pseudomonas sp.
0.125
-
NAD+
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
3 * 55000, SDS-PAGE Pseudomonas sp.
160000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. 13CM
-
-
-

Purification (Commentary)

Purification (Comment) Organism
14.2fold to apparent homogeneity, by hydrophobic chromatography and affinity chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.8
-
cell-free extract Pseudomonas sp.
12.4
-
14.2fold purified enzyme Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-dimethylaminobutyraldehyde + NAD+
-
Pseudomonas sp. 4-dimethylaminobutanoate + NADH
-
?
4-dimethylaminobutyraldehyde + NAD+
-
Pseudomonas sp. 13CM 4-dimethylaminobutanoate + NADH
-
?
4-N-trimethylaminobutyraldehyde + NAD+
-
Pseudomonas sp. 4-N-trimethylaminobutanoate + NADH
-
?
4-N-trimethylaminobutyraldehyde + NAD+
-
Pseudomonas sp. 13CM 4-N-trimethylaminobutanoate + NADH
-
?
additional information does not degradate 4-trimethylaminobutanol, betaine aldehyde, 4-aminobutyraldehyde, acetaldehyde, propionaldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde Pseudomonas sp. ?
-
?
additional information does not degradate 4-trimethylaminobutanol, betaine aldehyde, 4-aminobutyraldehyde, acetaldehyde, propionaldehyde, butyraldehyde, trimethylacetaldehyde, and 3-methylbutyraldehyde Pseudomonas sp. 13CM ?
-
?

Subunits

Subunits Comment Organism
trimer 3 * 55000, SDS-PAGE Pseudomonas sp.

Synonyms

Synonyms Comment Organism
4-N-trimethylaminobutyraldehyde dehydrogenase
-
Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
the enzyme is rapidly inactivated at temperatures above 35°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 9 the enzyme is completely inactivated at pH 9.6 Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
additional information does not react with NADP+ Pseudomonas sp.
NAD+ is specific for NAD+ Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
5.5