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Literature summary for 1.2.1.47 extracted from
- Carnitine biosynthesis. Purification of 4-N-trimethylaminobutyraldehyde dehydrogenase from beef liver (1980), J. Biol. Chem., 255, 1146-1151.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Disulfiram | - |
Bos taurus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00417 | - |
4-trimethylaminobutyraldehyde | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Bos taurus | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
3 * 55000, SDS-PAGE | Bos taurus |
| 160000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-trimethylaminobutyraldehyde + NAD+ | Bos taurus | function in carnitine biosynthesis | 4-trimethylaminobutyrate + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using column chromatography on blue dextran-Sepharose and 5'-AMP-Sepharose | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.21 | - |
activity on propionaldehyde | Bos taurus |
| 5.1 | - |
activity on 4-trimethylaminobutyraldehyde | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 0.1% 2-mercaptoethanol, 20% glycerol, several months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-trimethylaminobutyraldehyde + NAD+ | - |
Bos taurus | 4-trimethylaminobutyrate + NADH + H+ | - |
? | |
| 4-trimethylaminobutyraldehyde + NAD+ | function in carnitine biosynthesis | Bos taurus | 4-trimethylaminobutyrate + NADH + H+ | - |
? | |
| acetaldehyde + NAD+ + H2O | low activity | Bos taurus | acetate + NADH + H+ | - |
? | |
| butyraldehyde + NAD+ | low activity | Bos taurus | butyrate + NADH | - |
? | |
| propionaldehyde + NAD+ | low activity | Bos taurus | propionate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 55000, SDS-PAGE | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
0.1% 2-mercaptoethanol, 20% glycerol, fairly stable, but storage for several weeks results in loss of most of the activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | 9.8 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 10.5 | pH 6.2: about 30% of activity maximum, pH 10.5: about 15% of activity maximum | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | absolute requirement for | Bos taurus | |
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Release 2023.1 (January 2023)
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