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Literature summary for 1.2.1.44 extracted from
- Characterization of a cinnamoyl-CoA reductase that is associated with stem development in wheat (2007), J. Exp. Bot., 58, 2011-2021.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | CCR enzyme activity is increased progressively along with lignin biosynthesis and stem maturity. During stem development, CCR1 mRNA levels remain high at elongation, heading, and milky stages in the wheat H4564 cultivar. High CCR1 mRNA levels and high CCR enzyme activity in wheat stem are correlated with a higher Klason lignin content and greater stem mechanical strength in the H4564 cultivar. Escherichia coli BL21(DE3) and BL21(DE3)pLysS strains produce a much higher amount of CCR1 protein compared with the RIL and RP strains | Triticum aestivum |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | CCR1 is present as a single-copy gene in the wheat genome. It groups together with other monocot CCR sequences while it diverges from CCR2. It may be involved in the regulation of lignin biosynthesis during stem maturity and may contribute to stem strength support in wheat | Triticum aestivum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into the pET-29b vector and expressed in Escherichia coli strains BL21(DE3), BL21(DE3)pLysS, RIL, and RP | Triticum aestivum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | CCR enzyme activity declines dramatically at the heading and milky stages in stems of the C6001 cultivar | Triticum aestivum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01437 | - |
caffeoyl-CoA | - |
Triticum aestivum |  |
| 0.06006 | - |
sinapoyl-CoA | - |
Triticum aestivum | |
| 0.07697 | - |
feruloyl-CoA | - |
Triticum aestivum | |
| 0.1824 | - |
5-hydroxyferuloyl-CoA | - |
Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | A8DNN6 | H4564 cultivar and C6001 cultivar | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration, to homogeneity | Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | lower expression | Triticum aestivum | - |
| additional information | undetectable expression in roots | Triticum aestivum | - |
| stem | highly expressed | Triticum aestivum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-hydroxyferuloyl-CoA + NADPH + H+ | - |
Triticum aestivum | 5-hydroxyferulic aldehyde + CoA + NADP+ | - |
? | |
| caffeoyl-CoA + NADPH + H+ | - |
Triticum aestivum | caffealdehyde + CoA + NADP+ | - |
? | |
| feruloyl-CoA + NADPH + H+ | best substrate, which indicates preferential biosynthesis of G-type lignin | Triticum aestivum | coniferaldehyde + CoA + NADP+ | - |
? | |
| sinapoyl-CoA + NADPH + H+ | - |
Triticum aestivum | sinapaldehyde + CoA + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Triticum aestivum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CCR1 | - |
Triticum aestivum |
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Release 2023.1 (January 2023)
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