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Literature summary for 1.2.1.44 extracted from
- Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic pools (2006), J. Exp. Bot., 57, 1399-1411.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| for generation of RNAi constructs and for tomato transformation through Agrobacterium tumefaciens, into the pGEX4T-1 vector for expression in Escherichia coli BL21 cells | Solanum lycopersicum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36800 | - |
predicted molecular mass for CCR1 and CCR2 | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cinnamaldehyde + CoA + NADP+ | Solanum lycopersicum | - |
cinnamoyl-CoA + NADPH + H+ | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum lycopersicum | Q4U1I4 | - |
- |
| Solanum lycopersicum | Q4U1I5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant proteins are purified on a glutathione resin | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Solanum lycopersicum | - |
| leaf | - |
Solanum lycopersicum | - |
| stem | - |
Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | caffealdehyde + CoA + NADP+ | - |
? | |
| cinnamaldehyde + CoA + NADP+ | - |
Solanum lycopersicum | cinnamoyl-CoA + NADPH + H+ | - |
? | |
| coumaroyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | coumaraldehyde + CoA + NADP+ | - |
? | |
| feruloyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | coniferaldehyde + CoA + NADP+ | - |
? | |
| sinapoyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | sinapaldehyde + CoA + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CCR1 | - |
Solanum lycopersicum |
| CCR2 | - |
Solanum lycopersicum |
| cinnamoyl-CoA reductase | - |
Solanum lycopersicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
caffeoyl-CoA | CCR1 | Solanum lycopersicum | |
| 0.43 | - |
coumaroyl-CoA | CCR1 | Solanum lycopersicum | |
| 0.96 | - |
sinapoyl-CoA | CCR1 | Solanum lycopersicum | |
| 1.6 | - |
feruloyl-CoA | CCR2 | Solanum lycopersicum | |
| 14.2 | - |
feruloyl-CoA | CCR1 | Solanum lycopersicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
activity assay | Solanum lycopersicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Solanum lycopersicum | |
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Release 2023.1 (January 2023)
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