Cloned (Comment) | Organism |
---|---|
two genes AtCCR1 and AtCCR2, localised on chromosome 1, AtCCR1 is associated with lignin biosynthesis during development, AtCCR2 is associated with the hypersensitive response to pathogen attack | Arabidopsis thaliana |
two recombinant proteins expressed in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00098 | - |
feruloyl-CoA | recombinant fusion protein GST-AtCCR1 | Arabidopsis thaliana | |
0.0047 | - |
feruloyl-CoA | recombinant fusion protein GST-AtCCR2 | Arabidopsis thaliana | |
0.00516 | - |
caffeoyl-CoA | recombinant fusion protein GST-AtCCR2 | Arabidopsis thaliana | |
0.00632 | - |
sinapoyl-CoA | recombinant fusion protein GST-AtCCR1 | Arabidopsis thaliana | |
0.0125 | - |
caffeoyl-CoA | recombinant fusion protein GST-AtCCR1 | Arabidopsis thaliana | |
0.0307 | - |
sinapoyl-CoA | recombinant fusion protein GST-AtCCR2 | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36600 | - |
AtCCR2, predicted from gene sequence | Arabidopsis thaliana |
37500 | - |
AtCCR1, predicted from gene sequence | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant fusion proteins GST-AtCCR1 and GST-AtCCR2, expressed in Escherichia coli | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | AtCCR1, not AtCCR 2, northern blot analysis | Arabidopsis thaliana | - |
leaf | AtCCR1, not AtCCR2, northern blot analysis | Arabidopsis thaliana | - |
leaf | strong and transient increase of AtCCR2 mRNA after inoculation with Xanthomonas campestris pv. campestris, northern blot analysis | Arabidopsis thaliana | - |
stem | AtCCR1, not AtCCR2, northern blot analysis | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
caffeoyl-CoA + NADPH | - |
Arabidopsis thaliana | caffeic aldehyde + CoA + NADP+ | - |
? | |
cinnamoyl-CoA + NADPH | - |
Arabidopsis thaliana | cinnamaldehyde + CoA + NADP+ | - |
? | |
feruloyl-CoA + NADPH | - |
Arabidopsis thaliana | ferulic aldehyde + CoA + NADP+ | - |
? | |
sinapoyl-CoA + NADPH | - |
Arabidopsis thaliana | sinapic aldehyde + CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CCR | ambiguous | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
caffeoyl-CoA | recombinant fusion protein GST-AtCCR2 | Arabidopsis thaliana | |
0.0363 | - |
caffeoyl-CoA | recombinant fusion protein GST-AtCCR1 | Arabidopsis thaliana | |
0.0372 | - |
feruloyl-CoA | recombinant fusion protein GST-AtCCR2 | Arabidopsis thaliana | |
0.038 | - |
feruloyl-CoA | recombinant fusion protein GST-AtCCR1 | Arabidopsis thaliana | |
0.0583 | - |
sinapoyl-CoA | recombinant fusion protein GST-AtCCR2 | Arabidopsis thaliana | |
0.0612 | - |
sinapoyl-CoA | recombinant fusion protein GST-AtCCR1 | Arabidopsis thaliana |