Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 50% inhibition at 1 mM | Escherichia coli | |
Co2+ | 50% inhibition at 2.5 mM | Escherichia coli | |
Cu2+ | 75% inhibition at 0.1 mM | Escherichia coli | |
iodoacetamide | 40% inhibition at 1 mM | Escherichia coli | |
iodoacetate | 15% inhibition at 1 mM | Escherichia coli | |
L-proline | 25% inhibition at 50 mM | Pseudomonas aeruginosa | |
Ni2+ | 100% inhibition at 0.1 mM | Escherichia coli | |
p-chloromercuribenzoate | 100% inhibition at 1 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
SDS-PAGE, monomeric form | Escherichia coli |
189000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.84 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 6 months | Escherichia coli |
-70°C, 10% glycerol | Escherichia coli |
0-2°C, 2 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate 5-semialdehyde + phosphate + NADP+ | - |
Escherichia coli | L-5-glutamyl phosphate + NADPH | - |
? | |
L-glutamate 5-semialdehyde + phosphate + NADP+ | - |
Pseudomonas aeruginosa | L-5-glutamyl phosphate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 47000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
rapidly inactivated above, pH 7.0, 30 min, stable below | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0 and pH 8.5: about 20% of activity maximum | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | stable for at least 10 min | Escherichia coli |
7 | - |
long periods | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli | |
NADPH | - |
Pseudomonas aeruginosa |