Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli CodonPlus (DE3)-RIPL cells | Pyrobaculum ferrireducens |
Crystallization (Comment) | Organism |
---|---|
apoenzyme and in complex with isobutyraldehyde and/or NADP+, capillary counterdiffusion method | Pyrobaculum ferrireducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15 | - |
glyceraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
20 | - |
Isobutyraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
21 | - |
propionaldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum ferrireducens | G7VCG0 | - |
- |
Pyrobaculum ferrireducens 1860 | G7VCG0 | - |
- |
Purification (Comment) | Organism |
---|---|
Source 30Q column chromatography and Superdex 200 gel filtration | Pyrobaculum ferrireducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyceraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens | glycerate + NADPH + H+ | - |
ir | |
glyceraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens 1860 | glycerate + NADPH + H+ | - |
ir | |
isobutyraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens | isobutyrate + NADPH + H+ | - |
ir | |
isobutyraldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens 1860 | isobutyrate + NADPH + H+ | - |
ir | |
additional information | no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde | Pyrobaculum ferrireducens | ? | - |
? | |
additional information | no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde | Pyrobaculum ferrireducens 1860 | ? | - |
? | |
propionaldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens | propanoate + NADPH + H+ | - |
ir | |
propionaldehyde + NADP+ + H2O | - |
Pyrobaculum ferrireducens 1860 | propanoate + NADPH + H+ | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
AlDHPyr1147 | - |
Pyrobaculum ferrireducens |
NADP-dependent aldehyde dehydrogenase | - |
Pyrobaculum ferrireducens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Pyrobaculum ferrireducens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 85 | - |
Pyrobaculum ferrireducens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
glyceraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
2.9 | - |
propionaldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
4.1 | - |
Isobutyraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Pyrobaculum ferrireducens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme is strictly specific for NADP+ | Pyrobaculum ferrireducens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
glyceraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
0.14 | - |
propionaldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens | |
0.2 | - |
Isobutyraldehyde | at pH 8.8 and 60°C | Pyrobaculum ferrireducens |