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Literature summary for 1.2.1.4 extracted from

  • Bezsudnova, E.Y.; Petrova, T.E.; Artemova, N.V.; Boyko, K.M.; Shabalin, I.G.; Rakitina, T.V.; Polyakov, K.M.; Popov, V.O.
    NADP-dependent aldehyde dehydrogenase from archaeon Pyrobaculum sp.1860: structural and functional features (2016), Archaea, 2016, 9127857.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli CodonPlus (DE3)-RIPL cells Pyrobaculum ferrireducens

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and in complex with isobutyraldehyde and/or NADP+, capillary counterdiffusion method Pyrobaculum ferrireducens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
15
-
glyceraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
20
-
Isobutyraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
21
-
propionaldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens

Organism

Organism UniProt Comment Textmining
Pyrobaculum ferrireducens G7VCG0
-
-
Pyrobaculum ferrireducens 1860 G7VCG0
-
-

Purification (Commentary)

Purification (Comment) Organism
Source 30Q column chromatography and Superdex 200 gel filtration Pyrobaculum ferrireducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyceraldehyde + NADP+ + H2O
-
Pyrobaculum ferrireducens glycerate + NADPH + H+
-
ir
glyceraldehyde + NADP+ + H2O
-
Pyrobaculum ferrireducens 1860 glycerate + NADPH + H+
-
ir
isobutyraldehyde + NADP+ + H2O
-
Pyrobaculum ferrireducens isobutyrate + NADPH + H+
-
ir
isobutyraldehyde + NADP+ + H2O
-
Pyrobaculum ferrireducens 1860 isobutyrate + NADPH + H+
-
ir
additional information no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde Pyrobaculum ferrireducens ?
-
?
additional information no activity with phenylacetaldehyde, chlorobenzaldehyde, betaine aldehyde and succinic semialdehyde Pyrobaculum ferrireducens 1860 ?
-
?
propionaldehyde + NADP+ + H2O
-
Pyrobaculum ferrireducens propanoate + NADPH + H+
-
ir
propionaldehyde + NADP+ + H2O
-
Pyrobaculum ferrireducens 1860 propanoate + NADPH + H+
-
ir

Synonyms

Synonyms Comment Organism
AlDHPyr1147
-
Pyrobaculum ferrireducens
NADP-dependent aldehyde dehydrogenase
-
Pyrobaculum ferrireducens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Pyrobaculum ferrireducens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 85
-
Pyrobaculum ferrireducens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
glyceraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
2.9
-
propionaldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
4.1
-
Isobutyraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.5
-
Pyrobaculum ferrireducens

Cofactor

Cofactor Comment Organism Structure
NADP+ the enzyme is strictly specific for NADP+ Pyrobaculum ferrireducens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.08
-
glyceraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
0.14
-
propionaldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens
0.2
-
Isobutyraldehyde at pH 8.8 and 60°C Pyrobaculum ferrireducens