Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | absolutely dependent on | Proteus vulgaris | |
NH4+ | Km: 3.7 mM | Proteus vulgaris |
Crystallization (Comment) | Organism |
---|---|
- |
Proteus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
propanal | - |
Proteus vulgaris | |
0.03 | - |
isopentanal | - |
Proteus vulgaris | |
0.04 | - |
acetaldehyde | - |
Proteus vulgaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | can partially replace NH4+ in activation, Km: 16 mM | Proteus vulgaris | |
Rb+ | can partially replace NH4+ in activation, Km: 13 mM | Proteus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
gel filtration | Proteus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Proteus vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.6 | - |
- |
Proteus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NADP+ + H2O | 131% of the activity with isovaleraldehyde | Proteus vulgaris | acetate + NADPH + H+ | - |
? | |
benzaldehyde + NADP+ | 6% of the activity with isovaleraldehyde | Proteus vulgaris | benzoate + NADPH | - |
? | |
isopentanal + NADP+ + H2O | - |
Proteus vulgaris | isopentanoate + NADPH + H+ | - |
? | |
propanal + NADP+ + H2O | 181% of the activity with isovaleraldehyde | Proteus vulgaris | propionate + NADPH + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Proteus vulgaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | completely inactive with NAD+ | Proteus vulgaris |