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Literature summary for 1.2.1.4 extracted from
- Crystallization and properties of NADP-dependent aldehyde dehydrogenase from Gluconobacter melanogenus (1980), Agric. Biol. Chem., 44, 155-164.
No PubMed abstract available
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Gluconobacter oxydans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Gluconobacter oxydans |  |
| Hg2+ | - |
Acetobacter pasteurianus | |
| Hg2+ | - |
Gluconobacter oxydans | |
| monoiodoacetate | - |
Acetobacter aceti | |
| monoiodoacetate | - |
Gluconobacter oxydans | |
| PCMB | - |
Acetobacter aceti | |
| PCMB | - |
Gluconobacter oxydans | |
| Sn2+ | - |
Gluconobacter oxydans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
acetaldehyde | - |
Gluconobacter oxydans | |
| 0.03 | - |
NADP+ | - |
Gluconobacter oxydans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Acetobacter aceti | |
| Mg2+ | stimulates | Gluconobacter oxydans | |
| Mn2+ | - |
Acetobacter aceti | |
| Mn2+ | stimulates | Gluconobacter oxydans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
4 * 52000, SDS-PAGE | Gluconobacter oxydans |
| 148000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADP+ | Gluconobacter oxydans | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Acetobacter pasteurianus | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Gluconobacter cerinus | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Gluconacetobacter liquefaciens | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Gluconobacter sphaericus | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Gluconobacter gluconicus | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Gluconobacter roseus | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Gluconobacter albidus | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Frateuria aurantia | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Acetobacter ascendens | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Acetobacter orleanensis | enzyme is involved in assimilation of ethanol | ? | - |
? | |
| acetaldehyde + NADP+ | Acetobacter acetigenus | enzyme is involved in assimilation of ethanol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetobacter aceti | - |
IFO 3281, IFO 3283, IFO 3284 | - |
| Acetobacter acetigenus | - |
IFO 3278, IFO 3279, IFO 3280 | - |
| Acetobacter ascendens | - |
IFO 3188, IFO 3299 | - |
| Acetobacter orleanensis | - |
IFO 3170 | - |
| Acetobacter pasteurianus | - |
Acetobacter kuetzingianus, IFO 3222 | - |
| Acetobacter pasteurianus | - |
IFO 3191, IFO 3297 | - |
| Frateuria aurantia | - |
IFO 3248 | - |
| Gluconacetobacter liquefaciens | - |
IFO 12388 | - |
| Gluconobacter albidus | - |
IFO 3250, IFO 3251, IFO 3253 | - |
| Gluconobacter cerinus | - |
IFO 3262, IFO 3263, IFO 3264, IFO 3265, IFO 3266, IFO 3267, IFO 3268, IFO 3269, IFO 3270 | - |
| Gluconobacter gluconicus | - |
IFO 3171, IFO 3285, IFO 3286 | - |
| Gluconobacter oxydans | - |
IFO 3260 | - |
| Gluconobacter oxydans | - |
IFO 3293, IFO 12257, IFO 12258, IFO 3294 | - |
| Gluconobacter oxydans | - |
IFO 3189, IFO 3287 | - |
| Gluconobacter oxydans | - |
IFO 3171, IFO 3272, IFO 3274 | - |
| Gluconobacter oxydans | - |
IFO 3130, IFO 3172, IFO 3289, IFO 3290, IFO 3291, IFO 12528, var. alpha IFO 3254, var. alpha IFO 3255, var. alpha IFO 3256, var. alpha IFO 3257, var. alpha IFO 3258 | - |
| Gluconobacter oxydans | - |
IFO IFO 3462 | - |
| Gluconobacter oxydans | - |
IFO 3275, IFO 3276 | - |
| Gluconobacter oxydans | - |
IFO 3244 | - |
| Gluconobacter roseus | - |
- |
- |
| Gluconobacter sphaericus | - |
IFO 12467 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 148 | - |
- |
Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Gluconobacter oxydans | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Acetobacter pasteurianus | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Gluconobacter cerinus | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Gluconacetobacter liquefaciens | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Gluconobacter sphaericus | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Gluconobacter gluconicus | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Gluconobacter roseus | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Gluconobacter albidus | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Frateuria aurantia | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Acetobacter ascendens | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Acetobacter orleanensis | ? | - |
? | |
| acetaldehyde + NADP+ | enzyme is involved in assimilation of ethanol | Acetobacter acetigenus | ? | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Acetobacter aceti | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconobacter oxydans | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconobacter oxydans | acetate + NADPH + H+ | - |
ir | |
| acetaldehyde + NADP+ + H2O | - |
Acetobacter pasteurianus | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconobacter cerinus | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconacetobacter liquefaciens | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconobacter sphaericus | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconobacter gluconicus | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconobacter roseus | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Gluconobacter albidus | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Frateuria aurantia | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Acetobacter ascendens | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Acetobacter orleanensis | acetate + NADPH + H+ | - |
? | |
| acetaldehyde + NADP+ + H2O | - |
Acetobacter acetigenus | acetate + NADPH + H+ | - |
? | |
| butanal + NADP+ + H2O | - |
Acetobacter aceti | butyrate + NADPH + H+ | - |
? | |
| butanal + NADP+ + H2O | - |
Acetobacter pasteurianus | butyrate + NADPH + H+ | - |
? | |
| butanal + NADP+ + H2O | n-butanal, 2% of the activity with acetaldehyde | Gluconobacter oxydans | butyrate + NADPH + H+ | - |
ir | |
| formaldehyde + NADP+ + H2O | - |
Acetobacter pasteurianus | formate + NADPH + H+ | - |
? | |
| formaldehyde + NADP+ + H2O | 2% of the activity with acetaldehyde | Gluconobacter oxydans | formate + NADPH + H+ | - |
ir | |
| isobutanal + NADP+ | 19% of the activity with acetaldehyde | Gluconobacter oxydans | isobutyrate + NADPH | - |
ir | |
| propanal + NADP+ + H2O | - |
Acetobacter aceti | propionate + NADPH + H+ | - |
? | |
| propanal + NADP+ + H2O | - |
Acetobacter pasteurianus | propionate + NADPH + H+ | - |
? | |
| propanal + NADP+ + H2O | 102% of the activity with acetaldehyde | Gluconobacter oxydans | propionate + NADPH + H+ | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 4 * 52000, SDS-PAGE | Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Gluconobacter oxydans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | 4°C, stable | Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Acetobacter aceti | |
| NADP+ | - |
Gluconobacter oxydans | |
| NADP+ | - |
Acetobacter pasteurianus | |
| NADP+ | - |
Gluconobacter cerinus | |
| NADP+ | - |
Gluconacetobacter liquefaciens | |
| NADP+ | - |
Gluconobacter sphaericus | |
| NADP+ | - |
Gluconobacter gluconicus | |
| NADP+ | - |
Gluconobacter roseus | |
| NADP+ | - |
Gluconobacter albidus | |
| NADP+ | - |
Frateuria aurantia | |
| NADP+ | - |
Acetobacter ascendens | |
| NADP+ | - |
Acetobacter orleanensis | |
| NADP+ | - |
Acetobacter acetigenus | |
| NADP+ | completely inactive with NAD+ | Gluconobacter oxydans | |
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