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Literature summary for 1.2.1.38 extracted from
- N-Acetylglutamic gamma-semialdehyde dehydrogenase (Escherichia coli) (1970), Methods Enzymol., 17A, 255-260.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| gel filtration, enzyme unstable | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli |  |
| Cu2+ | - |
Escherichia coli | |
| Fe2+ | slight | Escherichia coli | |
| Mn2+ | slight | Escherichia coli | |
| N-ethylmaleimide | - |
Escherichia coli | |
| Ni2+ | slight | Escherichia coli | |
| p-hydroxymercuribenzoate | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
NADP+ | - |
Escherichia coli | |
| 0.6 | - |
N-acetyl-L-glumate 5-semialdehyde | - |
Escherichia coli | |
| 3 | - |
phosphate | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | Escherichia coli | enzyme of arginine biosynthesis | N-acetyl-5-glutamyl phosphate + NADPH | - |
? | |
| N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | Escherichia coli | enzyme of arginine biosynthesis | N-acetyl-5-glutamyl phosphate + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
W2D ATCC 25542 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, several weeks, pH 7.0, 0.1 M phosphate, 1.0 mM 2-mercaptoethanol, 0.1 mM EDTA | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | in the dehydrogenase reaction, arsenate can substitute for phosphate | Escherichia coli | N-acetyl-5-glutamyl phosphate + NADPH | - |
r | |
| N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | enzyme of arginine biosynthesis | Escherichia coli | N-acetyl-5-glutamyl phosphate + NADPH | - |
? | |
| N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate | enzyme of arginine biosynthesis | Escherichia coli | N-acetyl-5-glutamyl phosphate + NADPH | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | activity drops sharply below pH 9.0 and above 9.5 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | 4°C, 5 h | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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