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Literature summary for 1.2.1.32 extracted from

  • He, Z.; Davis, J.K.; Spain, J.C.
    Purification, characterization, and sequence analysis of 2-aminomuconic 6-semialdehyde dehydrogenase from Pseudomonas pseudoalcaligenes JS45 (1998), J. Bacteriol., 180, 4591-4595.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.32

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
 2-hydroxymuconate-6-semialdehyde
-
 Pseudomonas oleovorans
0.074
-
 NAD+
-
 Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 3 * 57000, SDS-PAGE, 3 * 57800, nucleotide sequence Pseudomonas oleovorans
57800
-
 3 * 57000, SDS-PAGE, 3 * 57800, nucleotide sequence Pseudomonas oleovorans
160000
-
 gel filtration Pseudomonas oleovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-aminomuconate-6-semialdehyde + NAD+ + H2O Pseudomonas oleovorans catabolism of nitrobenzene as sole source of carbon, nitrogen and energy 2-aminomuconate + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
 grown on nitrobenzene
-
Pseudomonas oleovorans JS45
-
 grown on nitrobenzene
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.62
-
-
 Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminomuconate-6-semialdehyde + NAD+ + H2O
-
 Pseudomonas oleovorans 2-aminomuconate + NADH
-
 ?
2-aminomuconate-6-semialdehyde + NAD+ + H2O catabolism of nitrobenzene as sole source of carbon, nitrogen and energy Pseudomonas oleovorans 2-aminomuconate + NADH
-
 ?
2-aminomuconate-6-semialdehyde + NAD+ + H2O
-
 Pseudomonas oleovorans JS45 2-aminomuconate + NADH
-
 ?
2-hydroxymuconate-6-semialdehyde + NAD+ + H2O
-
 Pseudomonas oleovorans 2-hydroxymuconate + NADH
-
 ?
acetaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans acetate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans JS45 acetate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans benzoate + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans JS45 benzoate + NADH + H+
-
 ?
crotonaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans crotonate + NADH
-
 ?
crotonaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans JS45 crotonate + NADH
-
 ?
formaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans formate + NADH
-
 ?
formaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans JS45 formate + NADH
-
 ?
hexaldehyde + NAD+ + H2O low activity Pseudomonas oleovorans n-hexanoic acid + NADH
-
 ?
n-butanal + NAD+ + H2O low activity Pseudomonas oleovorans n-butanoate + NADH + H+
-
 ?
propanal + NAD+ + H2O low activity Pseudomonas oleovorans n-propanoate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 57000, SDS-PAGE, 3 * 57800, nucleotide sequence Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 2-aminomuconic acid oxidation Pseudomonas oleovorans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas oleovorans