Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | Sinorhizobium meliloti |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sinorhizobium meliloti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
phosphonoacetaldehyde | at pH 7.5 and 30°C | Sinorhizobium meliloti | |
1 | - |
NAD+ | at pH 7.5 and 30°C | Sinorhizobium meliloti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55076 | - |
x * 55076, His-tagged enzyme, calculated from amino acid sequence | Sinorhizobium meliloti |
55100 | - |
x * 55100, His-tagged enzyme, SDS-PAGE | Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonoacetaldehyde + NAD+ | Sinorhizobium meliloti | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography, and gel filtration | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonoacetaldehyde + NAD+ | - |
Sinorhizobium meliloti | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55076, His-tagged enzyme, calculated from amino acid sequence | Sinorhizobium meliloti |
? | x * 55100, His-tagged enzyme, SDS-PAGE | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
phnY | - |
Sinorhizobium meliloti |
phosphonoacetaldehyde dehydrogenase | - |
Sinorhizobium meliloti |
PnAA dehydrogenase | - |
Sinorhizobium meliloti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sinorhizobium meliloti |