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Literature summary for 1.2.1.3 extracted from

  • Beretta, M.; Gorren, A.C.; Wenzl, M.V.; Weis, R.; Russwurm, M.; Koesling, D.; Schmidt, K.; Mayer, B.
    Characterization of the East Asian variant of aldehyde dehydrogenase-2: bioactivation of nitroglycerin and effects of Alda-1 (2010), J. Biol. Chem., 285, 943-952.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.3

Activating Compound

Activating Compound Comment Organism Structure
Alda-1 i.e. N-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamide, 0.01 mM Alda-1 increases acetaldehyde oxidation by wild type ALDH2 and the Asian variant E487K approximately 1.5 and 6fold, respectively. Alda-1 stimulates established ALDH2 activities by improving NAD+ binding but does not improve the nitroglycerin binding affinity of the Asian variant E487K Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E487K the East Asian variant results in very low aldehyde dehydrogenase activity and catalyzes nitroglycerin denitration with about 7fold lower maximal rates than the wild type enzyme1 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Alda-1 0.01 mM Alda-1 inhibits the apparent formaldehyde oxidation activity of the wild type enzyme by about 90% Homo sapiens
NAD+ pronounced inhibition occurs at above 5 mM Homo sapiens
nitroglycerin exposure of ALDH2 to nitroglycerin results in mechanism-based oxidative inactivation of the enzyme that is partially reversed by dithiothreitol Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
formaldehyde + NAD+ + H2O
-
 Homo sapiens formate + NADH + H+
-
 ?
nitroglycerin + NAD+ + H2O
-
 Homo sapiens 1,2-glyceryl dinitrate + NO + NADH + H+
-
 ?
propionaldehyde + NAD+ + H2O
-
 Homo sapiens propionate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase-2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens