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Literature summary for 1.2.1.3 extracted from

  • Nakamura, T.; Ichinose, H.; Wariishi, H.
    Cloning and heterologous expression of two aryl-aldehyde dehydrogenases from the white-rot basidiomycete Phanerochaete chrysosporium (2010), Biochem. Biophys. Res. Commun., 394, 470-475.
    View publication on PubMed
Search for more literature for 1.2.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53300
-
 isoform ALDH1, calculated from amino acid sequence Phanerodontia chrysosporium
55400
-
 isoform ALDH2, calculated from amino acid sequence Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-
Phanerodontia chrysosporium ATCC 34541
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His Gravi-Trap column chromatography Phanerodontia chrysosporium

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Phanerodontia chrysosporium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4,5-trimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4,5-trimethoxybenzoic acid + NADH + H+
-
 ?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4-dihydroxybenzoic acid + NADH + H+
-
 ?
3,4-dimethoxy-5-hydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4-dimethoxy-5-hydroxybenzoic acid + NADH + H+
-
 ?
3,4-dimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 3,4-dimethoxybenzoic acid + NADH + H+
-
 ?
4-hydroxy-3,5-dimethoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 4-hydroxy-3,5-dimethoxybenzoic acid + NADH + H+
-
 ?
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 4-hydroxy-3-methoxybenzoic acid + NADH + H+
-
 ?
4-hydroxybenzaldehyde + NAD+ + H2O
-
 Phanerodontia chrysosporium 4-hydroxybenzoic acid + NADH + H+
-
 ?
benzaldehyde + NAD+ + H2O best substrate, isoform ALDH1 shows 2fold greater catalytic activity against benzaldehyde than against other substrates, while isoform ALDH2 shows no substrate preferences Phanerodontia chrysosporium benzoate + NADH + H+
-
 ?
vanillin + NAD+ + H2O
-
 Phanerodontia chrysosporium vanillic acid + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
ALDH1 isoform Phanerodontia chrysosporium
ALDH2 isoform Phanerodontia chrysosporium
aryl-aldehyde dehydrogenases
-
 Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Phanerodontia chrysosporium

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 enzymatic reactions at pH 4.0 show only 5-10% of the maximum activities observed at pH 10.0 Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Phanerodontia chrysosporium

pI Value

Organism Comment pI Value Maximum pI Value
Phanerodontia chrysosporium isoform ALDH1, calculated from amino acid sequence
-
 5.9
Phanerodontia chrysosporium isoform ALDH2, calculated from amino acid sequence
-
 6.1

Expression

Organism Comment Expression
Phanerodontia chrysosporium both ALDH isoforms are translationally up-regulated in response to exogenous addition of vanillin up