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Literature summary for 1.2.1.3 extracted from
- Two aldehyde dehydrogenases from human liver. Isolation via affinity chromatography and characterization of the isozymes (1977), Biochim. Biophys. Acta, 483, 35-45.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing causes loss of activity | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Disulfiram | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
acetaldehyde | isoenzyme 1 | Homo sapiens | |
| 0.0007 | - |
propanal | isoenzyme 2, pH 7.0 | Homo sapiens | |
| 0.0012 | - |
propanal | isoenzyme 2, pH 9.5 | Homo sapiens | |
| 0.0024 | - |
acetaldehyde | isoenzyme 2, pH 9.5 | Homo sapiens | |
| 0.003 | - |
acetaldehyde | isoenzyme 2, pH 7.0 | Homo sapiens | |
| 0.008 | - |
NAD+ | isoenzyme 1, pH 9.5 | Homo sapiens | |
| 0.011 | - |
propanal | isoenzyme 2, pH 9.5 | Homo sapiens | |
| 0.04 | - |
NAD+ | isoenzyme 1, pH 7.0 | Homo sapiens | |
| 0.07 | - |
NAD+ | isoenzyme 2, pH 7.0 and pH 9.5 | Homo sapiens | |
| 0.12 | - |
acetaldehyde | isoenzyme 1, pH 9.5 | Homo sapiens | |
| 1 | - |
propanal | isoenzyme 1, pH 7.0 | Homo sapiens | |
| 2.5 | - |
acetaldehyde | isoenzyme 1, pH 7.0 | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54200 | - |
4 * 54200, isoenzyme 2, SDS-PAGE | Homo sapiens |
| 54800 | - |
4 * 54800, isoenzyme 1, SDS-PAGE | Homo sapiens |
| 225000 | - |
enzyme 2, non-denaturing PAGE | Homo sapiens |
| 245000 | - |
enzyme 1, non-denaturing PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.58 | - |
isoenzyme 1 | Homo sapiens |
| 1.01 | - |
isoenzyme E2 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetaldehyde + NAD+ + H2O | - |
Homo sapiens | acetate + NADH + H+ | - |
? | |
| propanal + NAD+ + H2O | - |
Homo sapiens | propionate + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 54800, isoenzyme 1, SDS-PAGE | Homo sapiens |
| tetramer | 4 * 54200, isoenzyme 2, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
isoenzyme 1 | Homo sapiens |
| 9 | - |
above, isoenzyme 2 | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | pH 7.0: about 66% of maximal activity, pH 9.0: about 85% of maximal activity, isoenzyme 1 | Homo sapiens |
| 8 | 9.5 | pH 8.0: about 35% of maximal activity, above pH 9.0: optimum, isoenzyme 2 | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
Disulfiram | isoenzyme 1 | Homo sapiens | |
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