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Literature summary for 1.2.1.3 extracted from

  • Ryzlak, M.T.; Pietruszko, R.
    Purification and characterization of aldehyde dehydrogenase from human brain (1987), Arch. Biochem. Biophys., 255, 409-418.
    View publication on PubMed
Search for more literature for 1.2.1.3

Inhibitors

Inhibitors Comment Organism Structure
acetaldehyde 0.02 mM, substrate inhibition of isoenzyme I, no inhibition of isoenzyme II and III Homo sapiens
Disulfiram inhibition of isoenzyme III, isoenzyme I is extremely sensitive, isoenzyme II is totally insensitive Homo sapiens
Mg2+ isoenzyme III Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 3,4-dihydroxyphenyl acetaldehyde isoenzyme II Homo sapiens
0.0007
-
 indole 3-acetaldehyde isoenzyme I Homo sapiens
0.001
-
 indole 3-acetaldehyde isoenzyme III Homo sapiens
0.003
-
 3,4-dihydroxyphenyl acetaldehyde isoenzyme III Homo sapiens
0.004
-
 propanal isoenzyme III Homo sapiens
0.005
-
 glycolaldehyde isoenzyme I Homo sapiens
0.021
-
 glycolaldehyde isoenzyme II Homo sapiens
0.048
-
 acetaldehyde isoenzyme III Homo sapiens
0.11
-
 glycolaldehyde isoenzyme III Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm isoenzyme III Homo sapiens 5737
-
mitochondrion isoenzyme I and II Homo sapiens 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activation of isoenzyme I and II Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 4 * 54000, isoenzyme I, II and III, SDS-PAGE Homo sapiens
208000 210000 isoenzyme I, non-denaturing PAGE Homo sapiens
216000
-
 isoenzyme II, non-denaturing PAGE Homo sapiens
220000
-
 isoenzyme III, gel filtration Homo sapiens
236000
-
 isoenzyme III, non-denaturing PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.29
-
 isoenzyme I Homo sapiens
0.445
-
 isoenzyme III Homo sapiens
3
-
 isoenzyme II Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydroxyphenylacetaldehyde + NAD+ + H2O
-
 Homo sapiens 3,4-dihydroxyphenylacetate + NADH + H+
-
 ?
acetaldehyde + NAD+ + H2O
-
 Homo sapiens acetate + NADH + H+
-
 ?
glycolaldehyde + NAD+ + H2O
-
 Homo sapiens glycolate + NADH
-
 ?
indole-3-acetaldehyde + NAD+ + H2O
-
 Homo sapiens indole-3-acetate + NADH + H+
-
 ?
propanal + NAD+ + H2O
-
 Homo sapiens propionate + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 54000, isoenzyme I, II and III, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 reaction with indole 3-acetaldehyde, isoenzyme III Homo sapiens
8.5
-
 reaction with acetaldehyde and indole 3-acetaldehyde, isoenzyme II Homo sapiens
9
-
 reaction with acetaldehyde, isoenzyme III Homo sapiens
9
-
 reaction with indole 3-acetaldehyde, isoenzyme I Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens