Protein Variants | Comment | Organism |
---|---|---|
S74A | half-life of the mutant enzyme at 50°C is 1 min compared to the half-life of the native enzyme of 1 min. Mutation diminishes NAD+ binding, affecting both the on and the off rates, as well as the rate-limiting step. About 100fold higher Km-value for NAD+ | Rattus norvegicus |
S74C | about 70fold higher Km-value for NAD+ | Rattus norvegicus |
S74T | about 100fold higher Km-value for NAD+ | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADPribose | competitive | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
benzaldehyde | native enzyme | Rattus norvegicus | |
0.0001 | - |
p-nitrobenzaldehyde | native enzyme | Rattus norvegicus | |
0.0001 | - |
p-Methoxybenzaldehyde | native enzyme | Rattus norvegicus | |
0.0001 | - |
4-(dimethylamino)cinnamaldehyde | native enzyme | Rattus norvegicus | |
0.0002 | - |
benzaldehyde | mutant enzyme S74A | Rattus norvegicus | |
0.0003 | - |
4-(dimethylamino)cinnamaldehyde | mutant enzyme S74A | Rattus norvegicus | |
0.0004 | - |
acetaldehyde | native enzyme | Rattus norvegicus | |
0.0004 | - |
p-nitrobenzaldehyde | mutant enzyme S74A | Rattus norvegicus | |
0.0004 | - |
p-Methoxybenzaldehyde | mutant enzyme S74A | Rattus norvegicus | |
0.0007 | - |
propanal | native enzyme | Rattus norvegicus | |
0.0007 | - |
Butanal | native enzyme | Rattus norvegicus | |
0.0008 | - |
hexanal | native enzyme | Rattus norvegicus | |
0.0008 | - |
propanal | native enzyme and mutant enzyme S74A | Rattus norvegicus | |
0.0009 | - |
Glutaraldehyde | native enzyme | Rattus norvegicus | |
0.0009 | - |
propanal | mutant enzyme S74A | Rattus norvegicus | |
0.002 | - |
Butanal | mutant enzyme S74A | Rattus norvegicus | |
0.0024 | - |
hexanal | mutant enzyme S74A | Rattus norvegicus | |
0.0027 | - |
acetaldehyde | mutant enzyme S74A | Rattus norvegicus | |
0.003 | - |
Glutaraldehyde | mutant enzyme S74A | Rattus norvegicus | |
0.01 | - |
NAD+ | native enzyme, reaction with propanal | Rattus norvegicus | |
0.038 | - |
glycolaldehyde | native enzyme | Rattus norvegicus | |
0.077 | - |
glycolaldehyde | mutant enzyme S74A | Rattus norvegicus | |
0.178 | - |
formaldehyde | native enzyme | Rattus norvegicus | |
0.7 | - |
formaldehyde | mutant enzyme S74A | Rattus norvegicus | |
1.9 | - |
NAD+ | mutant enzyme S74A, reaction with propanal | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and mutant enzyme S74A | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Rattus norvegicus | acetate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Rattus norvegicus | benzoate + NADH + H+ | - |
? | |
butanal + NAD+ + H2O | - |
Rattus norvegicus | butyrate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | - |
Rattus norvegicus | formate + NADH | - |
? | |
glutaraldehyde + NAD+ + H2O | - |
Rattus norvegicus | glutarate + NADH | - |
? | |
glycolaldehyde + NAD+ + H2O | - |
Rattus norvegicus | glycolate + NADH | - |
? | |
hexanal + NAD+ + H2O | - |
Rattus norvegicus | hexanoate + NADH + H+ | - |
? | |
m-methoxybenzaldehyde + NAD+ + H2O | - |
Rattus norvegicus | m-methoxybenzoate + NADH + H+ | - |
? | |
p-(dimethylamino)cinnamaldehyde + NAD+ + H2O | - |
Rattus norvegicus | p-(dimethylamino)cinnamate + NADH | - |
? | |
p-nitrobenzaldehyde + NAD+ + H2O | - |
Rattus norvegicus | p-nitrobenzoate + NADH | - |
? | |
propanal + NAD+ + H2O | - |
Rattus norvegicus | propionate + NADH | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
mutant enzyme S74A and native enzyme, stable | Rattus norvegicus |
50 | - |
half-life of mutant enzyme S74A is 1 min, half-life of native enzyme is 4 min | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
ADPribose | native enzyme | Rattus norvegicus | |
6.8 | - |
ADPribose | mutant enzyme S74A | Rattus norvegicus |