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Literature summary for 1.2.1.28 extracted from

  • Chalmers, R.M.; Fewson, C.A.
    Purification and characterization of benzaldehyde dehydrogenase I from Acinetobacter calcoaceticus [published erratum appears in Biochem J 1990 Feb 1;265(6):931] (1989), Biochem. J., 263, 913-919.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.28

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.10 mM, 50% inhibition after less than 1 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
benzaldehyde substrate inhibition at concentrations below 0.005 mM, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
iodoacetamide 10 mM, 50% inhibition after less than 1 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
iodoacetate 10 mM, 50% inhibition after 11 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus
N-ethylmaleimide 10 mM, 50% inhibition after less than 1 min, benzaldehyde dehydrogenase I Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00057
-
 benzaldehyde
-
 Acinetobacter calcoaceticus
0.079
-
 NAD+
-
 Acinetobacter calcoaceticus
11.2
-
 K+
-
 Acinetobacter calcoaceticus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ or NH4+ or Rb+ required for maximal activity, maximum activity occurs at approx. 70 mM K+ Acinetobacter calcoaceticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, dehydrogenase I, SDS-PAGE Acinetobacter calcoaceticus
141000 219000 dehydrogenase I, gel filtration with different gels Acinetobacter calcoaceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzaldehyde + NAD+ + H2O Acinetobacter calcoaceticus
-
 benzoate + NADH + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
dehydrogenase I Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.4
-
-
 Acinetobacter calcoaceticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-bromobenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 2-bromobenzoate + NADH
-
 ?
2-fluorobenzaldehyde + NAD+ + H2O 41% of activity with benzaldehyde Acinetobacter calcoaceticus 2-fluorobenzoate + NADH + H+
-
 ?
2-hydroxybenzaldehyde + NAD+ + H2O 39% of activity with benzaldehyde Acinetobacter calcoaceticus 2-hydroxybenzoate + NADH + H+
-
 ?
2-methoxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 2-methoxybenzoate + NADH + H+
-
 ?
2-methylbenzaldehyde + NAD+ + H2O 13% of activity with benzaldehyde Acinetobacter calcoaceticus 2-methylbenzoate + NADH
-
 ?
3,4-dihydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 3,4-dihydroxybenzoate + NADH + H+
-
 ?
3-fluorobenzaldehyde + NAD+ + H2O 98% of activity with benzaldehyde Acinetobacter calcoaceticus 3-fluorobenzoate + NADH + H+
-
 ?
3-hydroxybenzaldehyde + NAD+ + H2O 222% of activity with benzaldehyde Acinetobacter calcoaceticus 3-hydroxybenzoate + NADH + H+
-
 ?
3-methoxy-4-hydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 3-methoxy-4-hydroxybenzoate + NADH + H+
-
 ?
3-methoxybenzaldehyde + NAD+ + H2O 235% of activity with benzaldehyde Acinetobacter calcoaceticus 3-methoxybenzoate + NADH
-
 ?
3-methylbenzaldehyde + NAD+ + H2O 119% of activity with benzaldehyde Acinetobacter calcoaceticus 3-methylbenzoate + NADH
-
 ?
4-fluorobenzaldehyde + NAD+ + H2O 210% of activity with benzaldehyde Acinetobacter calcoaceticus 4-fluorobenzoate + NADH + H+
-
 ?
4-hydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 4-hydroxybenzoate + NADH + H+
-
 ?
4-methoxy-3-hydroxybenzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus 4-methoxy-3-hydroxybenzoate + NADH + H+
-
 ?
4-methylbenzaldehyde + NAD+ + H2O 240% of activity with benzaldehyde Acinetobacter calcoaceticus 4-methylbenzoate + NADH
-
 ?
benzaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O benzaldehyde dehydrogenase I exhibits esterase activity, approx. 2% of dehydrogenase activity Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir
benzaldehyde + NAD+ + H2O also active with NADP+, see also EC 1.2.1.7 Acinetobacter calcoaceticus benzoate + NADH + H+
-
 ir
hexan-1-al + NAD+ + H2O
-
 Acinetobacter calcoaceticus hexanoate + NADH
-
 ?
octan-1-al + NAD+ + H2O
-
 Acinetobacter calcoaceticus octanoate + NADH
-
 ?
perillaldehyde + NAD+ + H2O
-
 Acinetobacter calcoaceticus perillic acid + NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 56000, dehydrogenase I, SDS-PAGE Acinetobacter calcoaceticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Acinetobacter calcoaceticus
NADP+ 6% activity of NAD+ Acinetobacter calcoaceticus