Protein Variants | Comment | Organism |
---|---|---|
R124L | results obtained under pre-steady conditions show that both Arg residues participate not only in methylmalonate semialdehyde binding via stabilizing interactions between the guanidinium groups and the carboxylate but also in the formation of an efficient MSDH-NAD+-methylmalonate semialdehyde ternary complex | Bacillus subtilis |
R301L | results obtained under pre-steady conditions show that both Arg residues participate not only in methylmalonate semialdehyde binding via stabilizing interactions between the guanidinium groups and the carboxylate but also in the formation of an efficient MSDH-NAD+-methylmalonate semialdehyde ternary complex | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value of CoA for R301L mutant enzyme under steady-state conditions with propionaldehyde is above 1 mM at pH 8.2 and 30°C | Bacillus subtilis | |
0.006 | - |
methylmalonate semialdehyde | R124L mutant enzyme, steady-state conditions, pH 8.2 and 30°C | Bacillus subtilis | |
0.023 | - |
methylmalonate semialdehyde | R301L mutant enzyme, steady-state conditions, pH 8.2 and 30°C | Bacillus subtilis | |
0.033 | - |
NAD+ | wild type MSDH, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.038 | - |
NAD+ | R124L mutant enzyme, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.06 | - |
NAD+ | R124L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.06 | - |
methylmalonate semialdehyde | wild type MSDH, steady-state conditions, pH 8.2 and 30°C | Bacillus subtilis | |
0.085 | - |
CoA | wild type MSDH, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.12 | - |
NAD+ | R301L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.12 | - |
CoA | wild type MSDH, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.33 | - |
CoA | R124L mutant enzyme, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.57 | - |
CoA | R124L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.62 | - |
CoA | R301L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
2.3 | - |
NAD+ | wild type MSDH, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
9.3 | - |
propionaldehyde | R124L mutant enzyme, steady-state conditions, pH 8.2 and 30°C | Bacillus subtilis | |
9.8 | - |
propionaldehyde | wild type MSDH, steady-state conditions, pH 8.2 and 30°C | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
MSDH purified by ammonium sulfate fractionation, 40-80%, and gel filtration on a ACA 34 resin | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methylmalonate semialdehyde + CoA + H2O + NAD+ | - |
Bacillus subtilis | propanoyl-CoA + HCO3- + NADH | - |
? | |
propionaldehyde + CoA + NAD+ | - |
Bacillus subtilis | propanoyl-CoA + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MSDH | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Kcat-value of CoA for R301L mutant enzyme under steady-state conditions with propionaldehyde is above 3 1/sec at pH 8.2 and 30°C | Bacillus subtilis | |
0.06 | - |
NAD+ | R124L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
0.14 | - |
NAD+ | R301L mutant enzyme, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
2.2 | - |
NAD+ | wild type MSDH, steady-state conditions with methylmalonate semialdehyde, pH 8.2 and 30°C | Bacillus subtilis | |
2.8 | - |
CoA | R124L mutant enzyme, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis | |
12.6 | - |
CoA | wild type MSDH, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.8 | - |
Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
CoA | R301L mutant enzyme, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis | |
8.5 | - |
CoA | R124L mutant enzyme, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis | |
150 | - |
CoA | wild type MSDH, steady-state conditions with propionaldehyde, pH 8.2 and 30°C | Bacillus subtilis |