Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoisopentanoate | competitive | Bacillus subtilis | |
iodoacetate | - |
Bacillus subtilis | |
L-2-Oxo-3-methylpentanoate | competitive | Bacillus subtilis | |
NEM | - |
Bacillus subtilis | |
PCMB | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
CoA | - |
Bacillus subtilis | |
1.66 | - |
L-alpha-keto-beta-methylvalerate | - |
Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methyl-2-oxobutanoate + CoA + NAD+ | specific for the L-isomer | Bacillus subtilis | 2-methylpropanoyl-CoA + CO2 + NADH | - |
? | |
4-methyl-2-oxopentanoate + CoA + NAD+ | - |
Bacillus subtilis | 3-methyl-butanoyl-CoA + CO2 + NADH | - |
? | |
L-alpha-keto-beta-methylvalerate + CoA + NAD+ | - |
Bacillus subtilis | ? | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | pH 6.0: about 25% of maximal activity, pH 7.5: about 45% of maximal activity | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
CoA | required | Bacillus subtilis | |
additional information | CoA and NAD+ can be replaced by potassium ferricyanide but the activity is much lower | Bacillus subtilis | |
additional information | enzyme probably identical with EC 1.2.4.4 | Bacillus subtilis | |
NAD+ | required | Bacillus subtilis |