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Literature summary for 1.2.1.24 extracted from

  • Swenby, N.P.; Picklo, M.J.
    The conserved R166 residue of ALDH5A (succinic semialdehyde dehydrogenase) has multiple functional roles (2009), Chem. Biol. Interact., 178, 70-74.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
R166A the mutant enzyme shows no activity towards acetaldehyde and decreased activity and higher KM for succinate semiacetaldehyde Rattus norvegicus
R166E the mutant enzyme shows almost no activity towards acetaldehyde and decreased activity and higher KM for succinate semiacetaldehyde Rattus norvegicus
R166H the mutant has KM for succinate semiacetaldehyde of 800fold greater than the wild type enzyme, while the VMAX for this mutant is 11.3fold less than wild type for succinate semiacetaldehyde Rattus norvegicus
R166K the mutant has KM for succinate semiacetaldehyde of 8fold greater than the wild type enzyme, while the VMAX for this mutant is 2.5fold less than wild type for succinate semiacetaldehyde Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0109
-
succinate semialdehyde mutant enzyme R166K, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
0.013
-
succinate semialdehyde wild type enzyme, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
0.021
-
NAD+ wild type enzyme, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
0.035
-
NAD+ mutant enzyme R166E, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
0.044
-
NAD+ mutant enzyme R166A, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
0.063
-
NAD+ mutant enzyme R166H, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
0.081
-
NAD+ mutant enzyme R166K, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
0.62
-
succinate semialdehyde mutant enzyme R166E, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
1.13
-
succinate semialdehyde mutant enzyme R166H, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
1.6
-
succinate semialdehyde mutant enzyme R166A, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
3.16
-
acetaldehyde mutant enzyme R166K, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
4.3
-
acetaldehyde wild type enzyme, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
4.5
-
acetaldehyde mutant enzyme R166H, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus
4.82
-
acetaldehyde mutant enzyme R166E, at 37°C, in 40 mM sodium phosphate, pH 7.4 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Rattus norvegicus acetate + NADH + H+
-
?
succinate semialdehyde + NAD+ + H2O
-
Rattus norvegicus succinate + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
ALDH5A
-
Rattus norvegicus
succinic semialdehyde dehydrogenase
-
Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8.2 with wild type enzyme, similar activity is observed with pH 7.4 and 8.2, only 10% of the pH 7.4 activity is observed when the pH is reduced to pH 6.0, substitution with a lysine removes the pH sensitivity whereas the R166H mutant enzyme has higher activity at pH 6.0 than at pH 8.0 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rattus norvegicus