Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 (DE3) cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
R166A | the mutant enzyme shows no activity towards acetaldehyde and decreased activity and higher KM for succinate semiacetaldehyde | Rattus norvegicus |
R166E | the mutant enzyme shows almost no activity towards acetaldehyde and decreased activity and higher KM for succinate semiacetaldehyde | Rattus norvegicus |
R166H | the mutant has KM for succinate semiacetaldehyde of 800fold greater than the wild type enzyme, while the VMAX for this mutant is 11.3fold less than wild type for succinate semiacetaldehyde | Rattus norvegicus |
R166K | the mutant has KM for succinate semiacetaldehyde of 8fold greater than the wild type enzyme, while the VMAX for this mutant is 2.5fold less than wild type for succinate semiacetaldehyde | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0109 | - |
succinate semialdehyde | mutant enzyme R166K, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
0.013 | - |
succinate semialdehyde | wild type enzyme, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
0.021 | - |
NAD+ | wild type enzyme, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
0.035 | - |
NAD+ | mutant enzyme R166E, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
0.044 | - |
NAD+ | mutant enzyme R166A, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
0.063 | - |
NAD+ | mutant enzyme R166H, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
0.081 | - |
NAD+ | mutant enzyme R166K, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
0.62 | - |
succinate semialdehyde | mutant enzyme R166E, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
1.13 | - |
succinate semialdehyde | mutant enzyme R166H, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
1.6 | - |
succinate semialdehyde | mutant enzyme R166A, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
3.16 | - |
acetaldehyde | mutant enzyme R166K, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
4.3 | - |
acetaldehyde | wild type enzyme, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
4.5 | - |
acetaldehyde | mutant enzyme R166H, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus | |
4.82 | - |
acetaldehyde | mutant enzyme R166E, at 37°C, in 40 mM sodium phosphate, pH 7.4 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Rattus norvegicus | acetate + NADH + H+ | - |
? | |
succinate semialdehyde + NAD+ + H2O | - |
Rattus norvegicus | succinate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH5A | - |
Rattus norvegicus |
succinic semialdehyde dehydrogenase | - |
Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8.2 | with wild type enzyme, similar activity is observed with pH 7.4 and 8.2, only 10% of the pH 7.4 activity is observed when the pH is reduced to pH 6.0, substitution with a lysine removes the pH sensitivity whereas the R166H mutant enzyme has higher activity at pH 6.0 than at pH 8.0 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus |