Protein Variants | Comment | Organism |
---|---|---|
N286E | engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde | Escherichia coli |
N286H | engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde | Escherichia coli |
N286T | engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde. Compared to L-lactaldehyde, N286T has a one-third lower Km value to glyceraldehyde. | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.27 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli | |
5.8 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli | |
7.5 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli | |
14.5 | - |
(S)-lactaldehyde | wild-type, pH 7.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25553 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.74 | - |
wild-type, pH 7.0, 37°C | Escherichia coli |
1.18 | - |
mutant N286H, pH 7.0, 37°C | Escherichia coli |
1.78 | - |
mutant N286H, pH 7.0, 37°C | Escherichia coli |
2.18 | - |
mutant N286H, pH 7.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-lactaldehyde + NAD+ + H2O | - |
Escherichia coli | (S)-lactate + NADH + 2 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AldA | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
1 h, 70% residual activity for mutant N286T | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
761.5 | - |
(S)-lactaldehyde | wild-type, pH 7.0, 37°C | Escherichia coli | |
1026 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli | |
1545 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli | |
1895 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.5 | - |
(S)-lactaldehyde | wild-type, pH 7.0, 37°C | Escherichia coli | |
206.1 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli | |
326.8 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli | |
808.1 | - |
(S)-lactaldehyde | mutant N286H, pH 7.0, 37°C | Escherichia coli |