Literature summary for 1.2.1.22 extracted from

Inoue, Y.; Watanabe, K.; Shimosaka, M.; Saikusa, T.; Fukuda, Y.; Murata, K.; Kimura, A.
Metabolism of 2-oxoaldehydes in yeasts. Purification and characterization of lactaldehyde dehydrogenase from Saccharomyces cerevisiae (1985), Eur. J. Biochem., 153, 243-247.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	slight activation	Saccharomyces cerevisiae
glutathione	slight activation	Saccharomyces cerevisiae

General Stability

General Stability	Organism
purified enzyme is extremely unstable, reactivation with halide ions e.g. Cl-, I-, Br-	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	-	Saccharomyces cerevisiae
Cu2+	-	Saccharomyces cerevisiae
Hg2+	-	Saccharomyces cerevisiae
additional information	iodoacetamide, Mn2+, Mg2+, Co2+, no effect at 5 mM	Saccharomyces cerevisiae
N-ethylmaleimide	-	Saccharomyces cerevisiae
Ni2+	-	Saccharomyces cerevisiae
p-chloromercuribenzoate	-	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.9	-	NAD+	-	Saccharomyces cerevisiae
10	-	L-lactaldehyde	-	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Br-	activates	Saccharomyces cerevisiae
Cl-	activates	Saccharomyces cerevisiae
I-	activates	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	gel filtration, SDS-PAGE	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.392	-	-	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
-20°C, pH 7.5, purified enzyme is extremely unstable, almost completely inactivated, reactivation with halide ions Cl-, I-, Br-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-lactaldehyde + NAD+ + H2O	-	Saccharomyces cerevisiae	(S)-lactate + NADH + H+	-	?
D-lactaldehyde + NAD+ + H2O	0.2% the activity of L-lactaldehyde reduction	Saccharomyces cerevisiae	D-lactate + NADH + H+	-	?
additional information	not: acetaldehyde, DL-glyceraldehyde, propionaldehyde, phenylpyruvate	Saccharomyces cerevisiae	?	-	?
additional information	specifically oxidizes L-lactaldehyde to L-lactate in presence of NAD+	Saccharomyces cerevisiae	?	-	?

Synonyms

Synonyms	Comment	Organism
NAD-dependent lactaldehyde dehydrogenase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
additional information	-	rapid decline in activity above 60°C	Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	pH 6.5, 10 min, 50% loss of activity	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Saccharomyces cerevisiae

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7.5	-	4°C, stable	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
additional information	NADP+ can not substitute for NAD+	Saccharomyces cerevisiae
NAD+	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)