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Literature summary for 1.2.1.19 extracted from

  • Jakoby, W.B.; Fredericks, J.
    Pyrrolidine and putrescine metabolism: gamma-aminobutyraldehyde dehydrogenase (1959), J. Biol. Chem., 234, 2145-2150.
    View publication on PubMed
Search for more literature for 1.2.1.19

General Stability

General Stability Organism
2-mercaptoethanol and NAD+, stabilization Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
 A.T.C.C. 13430
-
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using protamine, ammonium sulfate, acetone and column chromatography on DEAE-cellulose Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanal + NAD+ + H2O
-
 Pseudomonas fluorescens 4-aminobutanoate + NADH + H+
-
 ?
4-aminobutanal + NAD+ + H2O
-
 Pseudomonas sp. 4-aminobutanoate + NADH + H+
-
 ?
additional information
-
 Pseudomonas sp. ?
-
 ?
additional information not: DELTA1-piperidine, glutamic semialdehyde, succinic semialdehyde, malonic semialdehyde Pseudomonas fluorescens ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2
-
 80% loss of activity after overnight storage Pseudomonas fluorescens
50
-
 for 5 min, 63% activity recovered Pseudomonas fluorescens
50
-
 for 5 min, NAD+ present during heating, 100% activity recovered Pseudomonas fluorescens
60
-
 for 5 min, 33% activity recovered Pseudomonas fluorescens
60
-
 for 5 min, NAD+ present during heating, 53% activity recovered Pseudomonas fluorescens
70
-
 for 5 min, 3% activity recovered Pseudomonas fluorescens
70
-
 for 5 min, NAD+ present during heating, 16% activity recovered Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas fluorescens
NAD+
-
 Pseudomonas sp.