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Literature summary for 1.2.1.19 extracted from

  • Jakoby, W.B.
    Enzymes of gamma-aminobutyrate metabolism (bacterial) (1962), Methods Enzymol., 5, 765-778.
No PubMed abstract available

General Stability

General Stability Organism
NAD+ and sulfhydryl compounds are necessary for stabilization Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
4-aminobutanal
-
Pseudomonas fluorescens
0.04
-
NAD+
-
Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-aminobutanal + NAD+ + H2O Escherichia coli
-
4-aminobutanoate + NADH + H+
-
?
4-aminobutanal + NAD+ + H2O Pseudomonas fluorescens gamma-aminobutyrate metabolism 4-aminobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas fluorescens
-
A.T.C.C. 13430
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.6
-
purified enzyme Pseudomonas fluorescens

Storage Stability

Storage Stability Organism
2°C, overnight, purified enzyme, 20% loss of activity Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminobutanal + NAD+ + H2O
-
Escherichia coli 4-aminobutanoate + NADH + H+
-
?
4-aminobutanal + NAD+ + H2O
-
Pseudomonas fluorescens 4-aminobutanoate + NADH + H+
-
?
4-aminobutanal + NAD+ + H2O specific for Escherichia coli 4-aminobutanoate + NADH + H+
-
?
4-aminobutanal + NAD+ + H2O specific for Escherichia coli 4-aminobutanoate + NADH + H+
-
ir
4-aminobutanal + NAD+ + H2O gamma-aminobutyrate metabolism Pseudomonas fluorescens 4-aminobutanoate + NADH + H+
-
?
additional information 1-pyrroline is used as source of substrate, delta1-pyrroline and gamma-aminobutyraldehyde exist as an equilibrium mixture in aqueous solution with the former as the predominant species Pseudomonas fluorescens ?
-
?
additional information not: DELTA1-piperidine, glutamic semialdehyde, succinic semialdehyde, malonic semialdehyde Pseudomonas fluorescens ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-15
-
retains 70% of activity when stored for 2 weeks Pseudomonas fluorescens
2
-
80% loss of activity after overnight storage Pseudomonas fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Pseudomonas fluorescens

pH Range

pH Minimum pH Maximum Comment Organism
7 8.3 highly active between Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
additional information not NADP+ Pseudomonas fluorescens
NAD+
-
Escherichia coli
NAD+
-
Pseudomonas fluorescens